Multicellular living organisms and unmodified parts thereof and – Plant – seedling – plant seed – or plant part – per se – Higher plant – seedling – plant seed – or plant part
Reexamination Certificate
2000-12-01
2004-09-14
Kubelik, Anne (Department: 1638)
Multicellular living organisms and unmodified parts thereof and
Plant, seedling, plant seed, or plant part, per se
Higher plant, seedling, plant seed, or plant part
C536S024100, C800S287000, C435S320100, C435S419000
Reexamination Certificate
active
06791013
ABSTRACT:
FIELD OF THE INVENTION
The invention provides DNA sequences and constructs that are useful in genetic engineering of plants. More particularly, the invention provides an isolated DNA sequence encoding maize myo-inositol-1-phosphate synthase (MIP synthase) and novel regulatory sequences derived from the MIP synthase gene, that can be used to drive expression of a variety of nucleic acid sequences in embryo tissue of transgenic plants.
BACKGROUND OF THE INVENTION
Plant genetic engineering projects require access to a variety of genetic elements that are used to regulate transgene expression. A primary example is the promoter, which regulates initiation of transcription.
A need exists for a variety of promoters for use in genetic engineering of plants. In particular, a need exists for promoters that drive expression specifically in embryo tissue.
BRIEF DESCRIPTION OF THE SEQUENCES
SEQ ID NO:1 is the DNA sequence for maize MIP synthase.
SEQ ID NO:2 is the amino acid sequence for maize MIP synthase.
SEQ ID NO:3 is the DNA sequence for the embryo specific maize MIP synthase promoter.
SUMMARY OF THE INVENTION
The invention provides an isolated DNA molecule encoding maize MIP synthase.
In another of its aspects, the invention provides embryo specific maize MIP synthase promoters corresponding to or derived from SEQ ID NO:3.
In another of its aspects, the invention provides a DNA construct comprising, operatively linked in the 5′ to 3′ direction,
a) a maize MIP synthase promoter;
b) a DNA sequence of interest; and
c) a 3′UTR.
In another of its aspects, the invention provides a plasmid comprising a maize MIP synthase promoter, preferably bp 7-2064 of SEQ ID NO 3.
In another of its aspects, the invention provides a transformed plant comprising at least one plant cell that contains a DNA construct of the invention. The plant may be a monocot or dicot. Preferred plants are maize, rice, cotton and tobacco.
In another of its aspects, the invention provides seed or grain that contains a DNA construct of the invention.
REFERENCES:
patent: 6225529 (2001-05-01), Lappegard et al.
Nelson et al., Regualation of Cell-Specific Inositol Metabolism and Transport in Plant Salinity Tolerance, May 1998, The Plant Cell, vol. 10, No. 5, pp. 753-764.*
Maiti et al., Promoter/leader deletion analysis and plant expression vector with the figwort mosaic virus (FMV)..., 1997, Transgenic Research, vol. 6, pp. 143-156.*
Donal et al., Mutation of either G box or I box sequence profoundly affects expression from the Arabidopsis rbcS-1A promoter, 1990, The EMBO Journal, vol. 9, No. 6, pp. 1717-1726.*
Lopes et al., Analysis of sequence in the INO1 promoter that are involved in its regulation by phospholipid precursors, 1991, Nucleic Acid Research, vol. 19, No. 7, pp. 1687-1693.*
Leppegard, US-09-377-648-7.
Armstrong Katherine
Folkerts Otto
Hey Timothy D.
Hopkins Nicole L.
Smith Kelley A.
Dow AgroSciences LLC
Kubelik Anne
Ludwig Kenneth B.
Stuart Donald R.
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