Maize embryo-specific promoter compositions and methods for...

Chemistry: molecular biology and microbiology – Process of mutation – cell fusion – or genetic modification – Introduction of a polynucleotide molecule into or...

Reexamination Certificate

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C435S320100, C800S287000, C536S024100

Reexamination Certificate

active

07078234

ABSTRACT:
This invention provides an embryo specific promoter, e.g. derived from the 5′ regulatory region of an emb5 gene. Compositions comprising this promoter include DNA constructs useful for plant transformation and plants transformed with such DNA constructs. Further provided are methods for the expression of transgenes in plants using the embryo specific promoter sequences. The methods of the invention include the direct creation of transgenic plants with the emb5 promoter by genetic transformation, as well as by plant breeding methods. The sequences of the invention represent a valuable new tool for the creation of transgenic plants, preferably having one or more added beneficial characteristics.

REFERENCES:
Kim et al. 1994. Plant Mol Biol. vol. 24. p. 105-117.
Williams and Tsang, Nucleotide sequence of an abscisic acid-responsive, embryo-specific maize gene, Plant Physiology, 1992, 100:1067-1068.
Williams and Tsang, Analysis of multiple classes of abscisic acid-responsive genes during embryogenesis in Zea mays, Developmental Genetics, 1994, 15:415-424.

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