Maize dwarf mosaic virus cDNA

Details Maize dwarf mosaic virus cDNA Maize dwarf mosaic virus cDNA

1989-04-07

1995-06-27

Benzion, Gary

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

435 691, 4351723, 4352523, C12N 1500, C12N 700

Patent

active

054281440

ABSTRACT:
In the present invention, methods and materials are provided to isolate the coat protein gene from Maize Dwarf Mosaic Virus--Strain A (MDMVA). This gene (MDMVA-CP) is then incorporated in an expression cassette designed for suitable expression in a plant cell system. The resulting transformation vector is then introduced into maize callus to provide cross-protection to MDMV or related viral infections.

REFERENCES:
A. L. Eggenberger, et al., J. Gen. Virol. (1989) 70, 1853-1860 "The Nucleotide Sequence of a Soybean Mosaic Virus Coat Protein-Coding Region . . . ".
Julianne Nagel, et al., Virology, vol. 143, pp. 435-441 (1985) "Complementary DNA Cloning and Expression of the Papaya Ringspot Potyvirus Sequences Encoding Capsid Protein, . . . ".
D. D. Shukla, et al. J. Gen. Virol. (1989), 70, pp. 13-23, "A Novel Approach to the Serology of Potyviruses Involving Affinity-Purified Polyclonal Antibodies Directed Towards Virus-Specific . . . ".
D. D. Shukla, et al. J. Gen. Virol (1988) vol. 69 pp. 2703-2710, "Amino Acid Sequence Homology of Coat Proteins as a Basis for Identification . . . ".
D. D. Shukla, et al. Phytopathology, vol. 79, No. 2, pp. 223-229, "Taxonomy of Potyviruses Infecting Maize, Sorghum, and Sugarcane in Australia and the United States . . . ".
W. G. Dougherty, et al. Virology 171:356-364. "Molecular Genetic Analysis of a Plant Virus Polyprotein Cleavage Site: A Model".
W. G. Dougherty, et al., EMBO, vol. 7, No. 5:1281-1287., "Biochemical and Mutational Analysis of a Plant Virus Polyprotein Cleavage Site".
W. G. Dougherty, et al., Ann. Rev. Phytopathol. 26:123-143., "Expression and Function of Potyviral Gene Products".
Murphy J. F., et al. Poster presented at the Symposium on Viral Genes and Plant Pathogenesis held at Lexington, Kentucky on Oct. 16-17, 1989. No. 31.
Viral Genes and Plant pathogenesis. 1990 Pirone, T. P. and Shaw, J. G., editors, Published by Springer-Verlag. Reference to and restatement of above poster (No. 31). IN PRESS.
Nagel et al. (1985) Virology 143 pages 435-441.
von Breungarten et al. (1981). Phytopathology 71(1):pp. 36-41.
Abel etal. (1986). Science. vol. 232, pp. 738-743.
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Krutzsch (1983) "Methods in Enzymology" vol. 91 Ed by Hirs et al. Academic Press, N.Y. pp. 511-524.

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