Maize cytoplasmic male sterility type T (cms-T) mitochondria DNA

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid

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4353201, 536 231, 536 236, 536 243, C07H 2104, C12N 1570, C12Q 168

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056609833

ABSTRACT:
Reagents and test methods for rapidly and specifically testing maize plants for the presence of T-type cms are provided. The reagent includes a novel nucleic acid segment whose sequence is uniquely arranged in mitochondrial DNA of cms-T maize. The segment, designated TURF 2H3, was cloned and vectors comprising TURF 2H3 provided. Subclones having sequences specific to cms-T mitochondrial DNA and the DNA and deduced amino acid sequences of ORI3, which is unique to T-type cytoplasm, are also provided.

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