Maize Cyclo1 gene and promoter

Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives

Reexamination Certificate

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Details

C536S023100, C800S289000, C800S278000, C800S320100, C800S279000, C435S468000, C435S412000, C435S320100

Reexamination Certificate

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11327727

ABSTRACT:
The present invention provides compositions and methods for regulating expression of heterologous nucleotide sequences in a plant. Compositions include a novel nucleotide sequence for a root-preferred promoter for the gene encoding Cyclo1. A method for expressing a heterologous nucleotide sequence in a plant using the promoter sequences disclosed herein is provided. The method comprises stabling incorporating into the genome of a plant cell a nucleotide sequence operably linked to the root-preferred promoter of the present invention and regenerating a stably transformed plant that expresses the nucleotide sequence. The present invention also relates to isolated nucleic acids encoding plant cyclotides. The invention relates to the construction of a chimeric gene encoding all or a portion of the plant cyclotides, in sense or antisense orientation, wherein expression of the chimeric gene results in the production of altered levels of plant cyclotides in a transformed host cell.

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Whitelaw, et al., Maize Genomics Consortium, NCBI Accession No. BZ785735 (2003).
Database EMBL, OG2BU79TH ZM—0.7—1.5—KBZea maysgenomic clone ZMMBMa0757N13, genomic survey sequence, (2003), EM—PRO: CG363034.
Trabi, Manuela and Craik, David J., Circular proteins—no end in sight, Trends in Biochem. Sci., (2002), 27 (3):132-138.

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