Chemistry: molecular biology and microbiology – Spore forming or isolating process
Patent
1991-07-30
1992-05-26
Robinson, Douglas W.
Chemistry: molecular biology and microbiology
Spore forming or isolating process
43524021, 4352403, 43524031, C12N 506, C12N 508
Patent
active
051167530
ABSTRACT:
The addition of basic FGF-saporin mitotoxins selectively elminates fibroblastoids from human islets in culture and increases the ability of such islets to release insulin under basal and stimulated conditions. When such islets are attached to an extracellular matrix, in particular BCEM, the proliferation of islet cells is favored, and the ability of islets cultured in such manner to release insulin is further increased. Moreover, supplementation of the culture media with high glucose or insulin further improves the functioning of the human islets, resulting in augmented insulin release. Combinations of such procedures offer a novel approach towards the establishment of viable human islet cell monolayers for clinical and laboratory research.
REFERENCES:
Beattie et al., J. Clin. Endocrinol Metab 73(1): 93-98 (1991).
Beattie et al., Diabetes 39(8): 1002-1005 (1990).
Baird J. Andrew
Beattie Gillian M.
Hayek Alberto
Lappi Douglas A.
Robinson Douglas W.
The Salk Institute for Biological Studies
The Whittier Institute for Diabetes & Endocrinology
Witz Jean C.
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