Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process...
Patent
1996-10-07
1999-11-09
Naff, David M.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
435 701, 435177, 435182, 435325, 435363, 435382, 435395, 435396, 435400, 435401, 4352891, 4352972, 4352974, 435813, C12P 100, C12M 300, C12N 500, C12N 1104
Patent
active
059812117
ABSTRACT:
Methods of maintaining animal cells for product production, for supporting hepatocyte function and viability to treat a patient suffering from hepatic failure and for preserving tissue-specific function of mammalian cells are carried out with a bioreactor containing a feed and waste chamber and a cell chamber separated by a selectively permeable membrane. Within the cell chamber, a biocompatible contracted three-dimensional gel matrix entraps animal cells or genetic modifications thereof, and a liquid phase contains a concentrated solution of the cell product. The bioreactor uses only two chambers to achieve three distinct zones within the bioreactor. The bioreactor can be of either hollow fiber or flat-bed configuration. In the configuration using hollow fibers, the two fluid paths correspond to the cavity surrounding the hollow fibers (the extracapillary space), and to the lumens of the hollow fibers themselves. Both fluid paths have inlet and outlet ports. Communication between the two fluid paths is across the permeable medium--the hollow fiber material. To prepare a bioartificial liver, hepatocytes are inoculated into the hollow fibers in a solution which quickly forms a highly porous gel. The gel subsequently contracts, leaving an open channel within the hollow fiber adjacent to the gel core entrapped hepatocytes. This channel can be perfused with nutrient media for hepatocytes. The channel can also serve as a waste stream to remove toxins that the hepatocytes have modified to a water soluble form.
REFERENCES:
patent: 3186917 (1965-06-01), Gerhardt et al.
patent: 3734851 (1973-05-01), Matsumura
patent: 3821087 (1974-06-01), Knazek et al.
patent: 3875008 (1975-04-01), Yoshino et al.
patent: 3883393 (1975-05-01), Knazek et al.
patent: 3898158 (1975-08-01), Miller
patent: 3997396 (1976-12-01), DeLente
patent: 4184922 (1980-01-01), Knazek et al.
patent: 4200689 (1980-04-01), Knazek et al.
patent: 4206015 (1980-06-01), Knazek et al.
patent: 4220725 (1980-09-01), Knazek et al.
patent: 4225671 (1980-09-01), Puchinger et al.
patent: 4242459 (1980-12-01), Chick et al.
patent: 4242460 (1980-12-01), Chick et al.
patent: 4353888 (1982-10-01), Sefton
patent: 4378017 (1983-03-01), Kosugi et al.
patent: 4407957 (1983-10-01), Lim
patent: 4411866 (1983-10-01), Kanno
patent: 4495288 (1985-01-01), Jarvis, Jr. et al.
patent: 4537860 (1985-08-01), Tolbert et al.
patent: 4546083 (1985-10-01), Meyers et al.
patent: 4559304 (1985-12-01), Kasai et al.
patent: 4582799 (1986-04-01), Jarvis, Jr.
patent: 4588407 (1986-05-01), Isono et al.
patent: 4603109 (1986-07-01), Lillo
patent: 4634447 (1987-01-01), Isono et al.
patent: 4639422 (1987-01-01), Geimer et al.
patent: 4640895 (1987-02-01), Davis
patent: 4643715 (1987-02-01), Isono et al.
patent: 4647536 (1987-03-01), Mosbach et al.
patent: 4649117 (1987-03-01), Familletti
patent: 4649118 (1987-03-01), Anderson
patent: 4661458 (1987-04-01), Berry et al.
patent: 4663286 (1987-05-01), Tsang et al.
patent: 4675002 (1987-06-01), Viles et al.
patent: 4692411 (1987-09-01), Ghose
patent: 4703108 (1987-10-01), Silver et al.
patent: 4743545 (1988-05-01), Torobin
patent: 4764471 (1988-08-01), Ripka
patent: 4861485 (1989-08-01), Fecondini
patent: 4923598 (1990-05-01), Schal
patent: 4955857 (1990-09-01), Shettigar
patent: 4975375 (1990-12-01), Haruta et al.
patent: 4997443 (1991-03-01), Walthall et al.
patent: 5043260 (1991-08-01), Jauregui
patent: 5595909 (1997-01-01), Hu et al.
Charles Lee et al., "Exchange Transfusion in Hepatic Coma: Report of a Case", Medical Journal of Australia, (Jan. 11, 1958), pp. 40-42.
B. Eiseman et al., "Heterologous Liver Perfusion in Treatment of Hepatic Failure". Annals of Surgery, (Sep. 1965), pp. 329-345.
Sen et al., "Use of Isolated Perfused Cadaveric LIver in the Manangement of Hepatic Failure" Surgery (May 1966), vol. 59, No. 5, pp. 774-781.
Trey et al., "Treatment of Hepatic Coma By Exchange Blood Transfusion", New England Journal of Medicine, (Mar. 1966), vol. 274, pp. 473-481.
Burnell et al., "Acute Hepatic Coma Treated By Cross-Circulation or Exchange Transfusion", New England Journal of Medicine, (Apr. 1967), vol. 276, pp. 935-943.
Sabin et al., "Treatment of Hepatic Coma in Cirrhosis by Plasmapheresis and Plasma Infusion", Annals of Internal Medicine, (Jan. 1968), vol. 68, pp. 1-7.
Wolf et al., "Bilirubin Conjugation By An Artificial Liver Composed Of Cultured Cells And Synthetic Capillaries", Trans.Amer.Soc.Artif. Int. Organs, (1975), vol. XXI, pp. 16-27.
Opolon et al., "Hepatic Failure Coma (HFC) Treated By Polyacrylonitrile Membrane (PAN) Hemodialysis (HD)", Irans.Amer.Soc.Artif.Int. Organs, (1976), vol. XXII, pp. 701-710.
Aden et al., "Controlled Synthesis of HBsAg in a Differentiated Human Liver Carcinoma-Derived Cell Line", Nature, (Dec. 1979), vol. 282, pp. 615-616.
Knowles et al., "Human Hepatocellular Carcinoma Cell Lines Secrete the Major Plasma Proteins and Hepatitis B Surface Antigen", Science, (Jul. 1980), vol. 209, pp. 497-499.
Rojkind et al., "Connective Tissue Biomatrix: Its Isolation and Utilization for Long-Term Cultures of Normal Rat Hepatocytes", Journal of Cell Biology, (Oct. 1980), vol. 87, pp. 255-263.
Rueff et al., "Acute Hepatic Necrosis and Fulminant Hepatic Failure", Gut, (1973), vol. 14, pp. 805-815.
Reid et al., Culturing Hepatocytes and Other Differentiated Cells, Hepatology, (1984), vol. 4, No. 3, pp. 548-559.
Antonio Martinez-Hernandez, "The Hepatic Extracellular Matrix", Laboratory Investigation, (1984) vol. 51, No. 1, pp. 57-74.
Jefferson et al., "Posttranscriptional Modulation of Gene Expression in Cultured Rat Hepatocytes", Molecular and Cellular Biology, (Sep. 1984), vol. 4, No. 9, pp. 1929-1934.
Friedman et al., "Hepatic Lipocytes: The Principal Collagen-Producing Cells of Normal Rat Liver", Proc. Natl. Acad. Sci. USA, (Dec. 1985), vol. 82, pp. 8681-8685.
Lie et al., "Successful Treatment of Hepatic Coma By A New Artificial Liver Device In The Pig", Res. Exp. Med., (1985), pp. 483-494.
O'Shea et al., "Encapsulation of Rat Islets of Langerhans Prolongs Xenograft Survival in Diabetic Mice", Diabetes, (Aug. 1986), vol. 35, pp. 943-946.
Ishihara et al., "Transport of Heparan Sulfate Into The Nuclei of Hepatocytes", Journal of Biological Chemistry, (1986), vol. 261, No. 29, pp. 13575-13580.
Geerts et al., "Immunogold Localization Of Procollagen III, Fibronectin and Heparan Sulfate Proteoglycan On Ultrathin Frozen Sections Of The Normal Rat Liver"., Histochemistry, (1986), pp. 355-362.
Sun et al., "Microencapsulated Hepatocytes As A Bioartificial Liver", Trans.Am.Soc.Artif.Intern.Organs, (1986), vol. XXXII, pp. 39-41.
Matsumura et al., "Hybrid Bioartificial Liver In Hepatic Failure: Preliminary Clinical Report" Surgery, (1987), pp. 99-103.
Spray et al., "Proteoglycans and Glycosaminoglycans Induce Gap Junction Synthesis and Function in Primary Liver Cultures", The Journal of Cell Biology, (Jul. 1987), vol. 105, pp. 541-551.
Busuttil et al., "The First 100 Liver Transplants At UCLA", Annals of Surgery, (1987), vol. 206, No. 4, pp. 387-402.
Sun et al., "Microencapsulated Hepatocytes: An In Vitro and In Vivo Study", Biomat., Art. Cells, Art. Org., (1987), 15(2), pp. 483-496.
Dich et al., "Long-Term Culture of Hepatocytes: Effect Of Hormones On Enzyme Activities and Metabolic Capacity"., Hepatology, (1988), vol. 8, No. 1, pp. 39-45.
Blake et al., "Accuracy of Death Certificates In The Diagnosis of Alcoholic Liver Cirrhosis", Alcoholism: Clinical and Experimental Research, (Jan.-Feb. 1988), vol. 12, No. 1, pp. 168-172.
Muzzarelli et al., "Biological Activity of Chitosan: Ultrastructural Study", Biomaterials, (May 1988), vol. 9, pp. 247-252.
Cai et al., "Microencapsulated Hepatocytes For Bioartificial Liver Support", Artificial Organs, (May 1988), vol. 12, No. 5, pp. 388-393.
Shellman et al., "Prognosis Of Patients With Cirrhosis And Chronic Liver Disease Admitted To The Medical Intensive Care Unit", Critical Care Medicine (Jul. 1988), vol. 16, No. 7., pp. 671-678.
Arenson et al., "Formation Of Extracellular Matrix In Normal Rat Liver: Lipocytes As A Major Source of Proteoglycan", Gastroenterol
Cerra Frank Bernard
Hu Wei-Shou
Nyberg Scott Lyle
Scholz Matthew Thomas
Shatford Russell A.
Naff David M.
Regents of the University of Minnesota
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