Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving virus or bacteriophage
Patent
1997-06-27
1999-03-02
Elliott, George C.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving virus or bacteriophage
435 6, 435 71, 435 721, 435 725, C12Q 168, C12Q 170, G01N 3353, G01N 33567
Patent
active
058769250
ABSTRACT:
The invention includes methods of isolating DNA and a protein encoded thereby, the protein being capable of binding to an antigen-bearing moiety. The methods comprise generating a DNA library expressing the protein in a virus vector. A magnetic label is added to cells expressing the antigen-bearing moiety and the cells are incubated with virus expressing the protein in the presence of an excess of unlabeled cells which do not express the antigen-bearing moiety to form a mixture, wherein the virus binds to the magnetically labeled cells. Cells having virus bound thereon are isolated from the mixture and DNA encoding the protein and the protein are obtained therefrom.
REFERENCES:
patent: 5338689 (1994-08-01), Yves et al.
patent: 5498538 (1996-03-01), Kay et al.
patent: 5658727 (1997-08-01), Barbas et al.
patent: 5663143 (1997-09-01), Ley et al.
Coia et al., 1996, J. Immunol. Methods, 192:13-23.
Hughes-Jones et al., 1994, British Journal of Haematology, 88:180-186.
Pereia et al., 1997, J. of Immunol. Methods, 203:11-24.
Siegel et al., 1997, J. of Immunol. Methds 206:73-85.
Winter et al., 1994, Annu. Rev. Immunol., 12:433-455.
DiaMed Ag Brochure, 1995, WorldWide pp. 1-6.
ID Microtyping System Brochure, 1995, Ortho Diagnostic System Inc., 14 pages.
Barbas, 1991, Proc. Natl. Acad. Sci. USA 88:7978-7982.
Barbas, 1995, "Synthetic human antibodies", Nature Medicine 1:837-839.
Barbas et al., 1991, Combinatorial Immunoglobulin Libraries On The Surface Of Phage (Phabs): Rapid Selection Of Antigen-Specific Fabs. Methods: A Companion To Methods In Enzymology 2:119-124.
Burton et al., 1994, "Human Antibodies from Combinatorial Libraries", Adv. Immunol. 57:191-280.
de Kruif et al., 1995, "Selection and Application of Human Single Chain Fv Antibody Fragments from a Semi-synthetic Phage Antibody Display Library with Designed CDR3 Regions", J. Mol. Biol.248:97-105.
Kang et al. 1991., Combinational Immunoglobulin Libraries On The Surface Of Phage (Phabs): Rapid Selection Of Antigen-Specific Fabs. Methods: A Companion To Methods In Enzymology 2:111-118.
Kretzschmar et al., 1995, Anal. Biochem. 224:413-419.
Lapierre et al., 1990, Transfusion 30:109-113.
Marks et al., 1991, "By-passign Immunization Human Antibodies from V-gene Libraries Displayed on Phage", J. Mol. Biol. 222:581-597.
Marks et al., 1993, "Human Antibody Fragments Specific for Human Blood group Antigens from a Phase Display Library", Bio/Technology 11:1145-1149.
Mollison et al., 1993, Blood Transfusion in Clin. Medicine, Oxford, Blackwell Scientific Publications--too voluminious to submit.
Roben et al., 1995, J. Immunol. 154:6437-6445.
Russell et al., 1993, "Retroviral vectors displaying functional antibody fragments", Nucl. Acids Res. 21:1081-1085.
Sambrook et al., 1989, Molecular Cloning: A Laboratory Manual, Cold Spring Harbor, NY--too voluminous to submit.
Siegel et al., 1994, Structural Analysis Of Red Cell Autoantibodies, Garratty (ed.) Immunobiology of Transfusion Medicine, Dekker, New York, NY, pp. 387-399.
Siegel, D., Submission entitled "A Plethora of Human Monoclonal Anti-Rh Antibodies Isolated Using Phage Display and Megnetically-Activated Cell Sorting", American Association of Blood Banks Annual Meeting, 1996, one page.
Silverman et al., 1995, J. Clin. Invest. 96:417-426.
Sternberg et al., 1995, "Display of peptides and proteins on the surface of bacteriophage .lambda.", Proc. Natl. Acad. Sci. USA 92:1609-1613.
Tomlinson et al., 1996, V Base Sequence Directory. MRC Center for Protein Engineering, Cambridge, UK--too voluminous to submit.
Walker, ed. 1993, Technical Manual, 11.sup.th Edition, Bethesda: American Association of Blood Banks --too voluminous to submit.
Siegel et al. (1994) Expression and characterization of recombinant anti-Rh(D) antibodies on filamentous phage: a model system for isolating human red blood cell antibodies by repertoire cloning. 83:2334-2344, Apr. 1994.
De Kruif et al. (1995) Rapid selection of cell subpopulation-specific human monoclonal antibodies from a synthetic phage display library. Proc. Natl. Acad. Sci. USA 92:3938-3942, Apr. 1995.
Russo et al. (1992) Studies with biotinylated RBC: (1) use of flow cytometry to determine posttransfusion survival and (2) isolation using streptavidin conjugated magnetic beads. Adv. Exp. Med. Biol. 326:101-107, 1992.
Elliott George C.
Schwartzman Robert
The Trustees of the University of Pennsylvania
LandOfFree
Magnetically activated cell sorting for production of proteins does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Magnetically activated cell sorting for production of proteins, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Magnetically activated cell sorting for production of proteins will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-421288