Harness for working animal – Hames – Fasteners
Reexamination Certificate
2002-09-27
2004-04-13
Lipman, Bernard (Department: 1713)
Harness for working animal
Hames
Fasteners
C524S431000
Reexamination Certificate
active
06718742
ABSTRACT:
The present invention relates to a method of making magnetic and paramagnetic particles which can be used in the separation of biomolecules or compounds from laboratory to industrial scale volumes.
In order to separate biomolecules such as nucleic acids from mixtures containing them it is known to use magnetic particles which are coated with a coating which will attach to the biomolecule which is to be separated. The particles are then added to the mixture and the particles attached to the biomolecules can be separated by use of a magnetic field.
The magnetic particles or beads must be of a size which enables them to remain in suspension in the liquids used. However if they are too small or contain fines or other similar particles they can stay in suspension and can be too slow to separate.
Known methods of making magnetic and paramagnetic particles include
1) Incorporation of magnetite (iron oxide, Fe
3
O
4
) inside porous agarose or cellulose followed by grinding or sieving to obtain a range of particle diameters, usually 1 to 10 microns.
2) Incorporation of magnetite inside silica followed by grinding or sieving to obtain a range of particle diameters, usually 1 to 10 microns.
3) Production of magnetite (less than 10 microns) by precipitation of iron salts, followed by surface coating with a silane or other functional group.
4) Coating a mono-disperse polystyrene bead (less than 10 microns) with submicron iron oxide, followed by another coating of polystyrene with or without functional groups.
5) Internal precipitation of iron oxide into a mono disperse polystyrene bead (less than 10 microns) followed by surface coating with functional groups.
Typically, magnetic beads have a diameter of less than 10 microns otherwise their sedimentation rates are too high under gravity for easy handling and the surface area too low to bind desired amount of the target molecule. Some larger beads such as agarose-magnetite may be up to 100 microns but they rely on being very porous to bind target molecules internally and separate out under gravity extremely quickly.
U.S. Pat. Nos. 4,695,392 and 5,091,206, WO 96/18731, EP 515484B1 disclose methods of forming and using such magnetic particles.
I have devised improved magnetic particles and a method of making them.
According to the invention there is provided a magnetic particle composition which comprises a magnetic material combined with negatively charged ion exchanger.
The invention also provides a method of forming a magnetic particle composition which method comprises contacting a magnetic material with a negatively charged ion exchanger.
The magnetic materials preferably have a diameter of less than ten microns and more preferably five microns or less.
The magnetic material can be any of the conventionally used magnetic materials such as magnetite, iron oxides, transition metal oxides or any ferro or paramagnetic material.
The ion exchanger can be porous or non porous and ion exchangers which can be used include polymethacrylate carboxy ion-exchangers, silica particles coated with a negative charge, cellulose or agarose with phosphate or sulphate groups or any negatively charged species.
The ion exchanger can be attached directly to the magnetic material e.g. by charge alone or it can be attached using a binding agent such as polymerised acrylic acid, or any agent that forms a coating or surface coat to aid cohesion of the materials. The particle may be further derivatised with functional groups such as carboxy, amine, imidazole etc.
The composition can readily be formed by mixing the components in powder form or by pre-mixing in aqueous or non-aqueous solutions with or without the binding agent.
The compositions of the invention i.e. the magnetic material combined with the ion exchanger, preferably have a diameter of between 0.5 microns to 1 mm and more preferably of 20 to 150 microns in diameter.
The ratio of magnetic particle to ion exchanger is not critical and can be varied in accordance with the application, typical ratios are from 5 to 50% (w/w) iron oxide.
Preferably a suspension of the particle composition of the invention in a liquid containing the material to be separated can be easily handled in conventional fluid handling and dispensing systems.
It is a feature of the present invention that the particle compositions provide faster magnetic separation or sedimentation under gravity in larger volumes without residual fines or particles remaining in suspension. The larger particles e.g 20 to 150 microns, also remain in suspension four longer compared to other magnetic beads of similar size made from other materials thus retaining effective mixing and binding kinetics.
The particles can be kept in suspension for dispensing with minimal agitation and also are preferably able to pass through standard pipette tips.
The compositions of the present invention can be used to separate biomolecules from mixtures containing them, for example they can be used to separate nucleic acids and to purify solutions or suspensions by removing impurities such as cell debris etc.
When the compositions are to be used to separate a biomolecule from a liquid containing the biomolecule the particle composition is contacted with a binding agent for the biomolecule for example biotinylated biomolecules may be isolated using Streptavidin coated magnetic particles.
The invention also provides a method for separating biomolecules from mixtures containing them which method comprises contacting a liquid suspension of the biomolecule with the magnetic particle composition as described above to form a suspension of the magnetic particle composition in which the particle composition binds to the biomolecule and applying a magnetic field to the suspension to separate out the magnetic particles having the biomolecule bound thereto.
The biomolecule can be separated from the magnetic particles by conventional means.
The method of the invention can be used to remove cellular debris or insoluble material without centrifugation or filtration.
For example, in the removal of cellular debris from a microbial, plasmid or plant DNA extraction, the particles can be used to rapidly remove unwanted contaminants leaving the target DNA in solution.
The invention is further described in the following examples.
REFERENCES:
patent: 5091206 (1992-02-01), Wang et al.
patent: 5714536 (1998-02-01), Ziolo et al.
patent: 5792445 (1998-08-01), Tournier et al.
patent: 196 24 426 (1998-01-01), None
Dann Dorfman Herrell & Skillman P.C.
DNA Research Innovations Limited
Lipman Bernard
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