Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1978-07-28
1980-12-23
Wiseman, Thomas G.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
260112R, 435176, 435181, C12Q 166, C07G 700
Patent
active
042411769
ABSTRACT:
The invention relates to a magnetic gel which is useful for immunoezymatic determinations.
Said gel consisting, for example, of polyacrylamideagarose and magnetic particles, coupled with an antibody, can be used for rapid determinations of the antigens contained in a biological liquid, the presence of magnetic particles permitting rapid separation of the gel from the reaction medium by means of a magnetic field and considerably shortening the time required for determinations.
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patent: 4108972 (1978-08-01), Dreyer
Chaplin et al., "Magnetic, Immobilized Derivatives of Enzymes," Chem. Abstracts, vol. 85, (1976), p. 203, Abs. No. 188415w.
Heden et al., "The Potential of Magnetic Separation of Biologically Active Proteins", Biotechmol. & Bioeng. Symp. No. 3, (1972), pp. 173-174.
Mosbach et al., Methods in Enzymology, vol. 44, Academic Press, New York, pp. 324-326.
Dunnill et al., "Purification of Enzymes Using Magnetic Bro-Affinity Materials", Biotech & Bioeng., vol. 16, (1974), pp. 987-990.
Guesdon, et al., "Magnetically Responsive Polyacrylamide Agnrose Beads for the Preparation of Immunoabsorbents", J. Immuno., Meth., vol. 21, (1978), pp. 59-63.
Guesdon, et al., "Dosages Immuno.-Enzymatiques des Ig E", Annales Medicales de Nancy, pp. 33-38.
Guesdon, et al., "Magnetic Enzyme Immunonssay for Measuring Human Ig E.", J. Allergy Chin. Immunol., vol. G1, No. 1, (1978), pp. 23-27.
Avrameas Stratis
Guesdon Jean-Luc
Etablissement declare d'Utilite Publique diti Institut Pasteur
Wiseman Thomas G.
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