Magnesium assay with calmagite or eriochrome black T reagents

Chemistry: analytical and immunological testing – Metal or metal containing – Present in biological fluids

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422 61, 436 79, G01N 3352

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active

043830439

ABSTRACT:
A colorimetric assay method and stabilized working reagents for the determination of magnesium in which an alkaline reagent containing a dye to complex magnesium reacts directly with the sample, requiring no protein-removal steps and no addition of substances to artificially correct for absorption differences of the metallized and free dye due to the presence of protein. The dye is either Calmagite or Eriochrome Black T. The reagent is buffered so that the pH is greater than about 9.0; the relative Van Slyke buffer value .beta.' of the reagent is greater than 1.

REFERENCES:
patent: 3754864 (1973-08-01), Gindler
patent: 3754865 (1973-08-01), Gindler
patent: 3798000 (1974-03-01), Helger
patent: 4205955 (1980-06-01), Sloat
S. A. Cohen, Clin. Chem., 26(6), 783 (1980).

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