Macromolecule recovery cassette

Details Macromolecule recovery cassette Macromolecule recovery cassette

1994-08-17

1996-07-23

Niebling, John

Chemistry: electrical and wave energy

Apparatus

Electrophoretic or electro-osmotic apparatus

204606, 204456, 204462, B01D 5702, G01N 2726

Patent

active

055386148

ABSTRACT:
An elution cassette is invented for macromolecule recovery from electrophoresis gels. The cassette comprises a frame (10), a front barrier (20), a back barrier (30), and a space inside the cassette (35) defined by the frame and the barriers. The barrier made of filter materials allows macromolecules to pass through while stopping gel debris. The back barrier is made of a membrane material allowing small ions to pass through but not macromolecules. When the cassette is inserted into an agarose gel and the electrophoresis is continued, macromolecules driven by an electric field migrate into the cassette through the front barrier and are stopped by the back barrier. The macromolecules contained in the space between the barriers can then be recovered.

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