Macromolecular environment control in specific receptor assays

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

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23230B, 424 12, 435177, 435178, 435810, 435 6, B12Q 166, C12N 1102, C12N 1110

Patent

active

042751497

ABSTRACT:
Method and compositions are provided for performing protein binding assays involving a homologous pair consisting of ligand and receptor for the ligand. The method employs a label conjugated to a member of said homologous pair and a uniformly dispersed discontinuous phase of discrete particles in a continuous aqueous phase, where the discrete particles create microenvironments which allow for discrimination between the label associated with the particle--in a discontinuous phase--and the label in the continuous phase.
Various conjugates and particles are provided which find use in the subject method.

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Wingard, et al., Applied Biochemistry and Bioengineering, vol. 1, Academic Press, NY, (1976), pp. 135-138.

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