Chemistry: molecular biology and microbiology – Treatment of micro-organisms or enzymes with electrical or... – Modification of viruses
Patent
1988-06-07
1992-03-24
Peet, Richard C.
Chemistry: molecular biology and microbiology
Treatment of micro-organisms or enzymes with electrical or...
Modification of viruses
435 712, 435 76, 435 91, 435169, 4351721, 43525235, 4353201, 435886, 536 27, 935 6, 935 9, 935 22, 935 29, 935 59, 935 60, 935 61, 935 66, 935 72, 935 75, C12N 121, C12N 1509, C12N 1576
Patent
active
050988379
ABSTRACT:
Spiramycin antibiotic biosynthetic genes of Streptomyces ambofaciens are provided by the present invention, in addition to a variety of recombinant DNA vectors. The genes also function in other macrolide producing organisms. The genes can be used to increase or otherwise alter the macrolide antibiotic-producing ability of an organism. The present invention also provides host strains comprising mutant spiramycin biosynthetic genes which can be used to generate novel antibiotics. Also provided is a method for preparing the mutant gene comprising mutating cloned spiramycin biosynthetic DNA by transposon mutagenesis with subsequent transformation into a macrolide-antibiotic producing host and homologous recombination into its genome, to generate stable mutant cell lines.
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Lacroix et al., Mutational Cloning in Streptomyces ambofaciens, Abstract from the 208th Meeting of the Genetical Society of Great Britain, Apr. 14-15, 1988, held at the John Innes Institute.
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PCT Publication Ser. No. WO 88-01646, Published Mar. 10, 1988.
Beckmann Robert J.
Cox Karen L.
Rao R. Nagaraja
Richardson Mark A.
Seno Eugene T.
Eli Lilly and Company
Hamilton Amy E.
Peet Richard C.
Whitaker Leroy
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