Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving virus or bacteriophage
Reexamination Certificate
2006-08-01
2006-08-01
Stucker, Jeffrey (Department: 1648)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving virus or bacteriophage
C435S007100, C435S962000, C436S518000
Reexamination Certificate
active
07083912
ABSTRACT:
A monoclonal antibody-based Dot-ELISA assay for the rapid detection of animal viruses such as avian influenza virus. The assay includes applying a specimen suspected of containing an animal virus on a porous membrane and treating the specimen with a solution of citric acid or lactic acid and a solution containing a mucolytic agent and a detergent. The treated specimen is then contacted with a primary monoclonal antibody for detecting the virus. If present, the primary moncolonal antibody bind with an antigen of the animal virus specimen. The specimen is contacted with an anti-monoclonal antibody conjugate (secondary antibody) and incubated to facilitate binding of the antigen-bound monoclonal antibody to the conjugate. The bound conjugate and antigen-bound monoclonal antibody is contacted with a coloring reagent to allow visual detection of the presence of the animal virus in the specimen.
REFERENCES:
patent: 4281061 (1981-07-01), Zuk et al.
patent: 4302444 (1981-11-01), Baxendale
patent: 4643578 (1987-02-01), Stern
patent: 4855408 (1989-08-01), Kuhn et al.
patent: 4942126 (1990-07-01), Slifkin
patent: 5173737 (1992-12-01), Mitchell et al.
patent: 5174993 (1992-12-01), Paoletti
patent: 5196514 (1993-03-01), Avakian et al.
patent: 5494801 (1996-02-01), Bogart et al.
patent: 5597735 (1997-01-01), Laszlo et al.
patent: 5773212 (1998-06-01), Figard
patent: 5916879 (1999-06-01), Webster
patent: 5989805 (1999-11-01), Reilly et al.
patent: 6033670 (2000-03-01), Bublot et al.
patent: 6265176 (2001-07-01), Lin et al.
patent: 6297062 (2001-10-01), Gombinski
patent: 6465171 (2002-10-01), Goebel et al.
patent: 6492166 (2002-12-01), Ferris et al.
patent: 6506385 (2003-01-01), Poston et al.
patent: 2003/0186285 (2003-10-01), Saito et al.
Schleicher & Schuell “Transfer and Immobilization of Proteins to S&S Solid Supports”, published by Schleicher & Schuell, Inc., 1981, p. 7.
Aldrich Catalog, 1001 West Saint Paul Ave, Milwaukee, WI, 53233, 1996, p. 1511.
James L. Bennington, Saunders Dictionary & Encyclopedia of Laboratory Medicine and Technology, Philadelphia, PA, W.B. Saunders Company, 1984, RB37, B45.
P. Tjssen, “Practice and Theory of Enzyme Immunoassays”, Laboratory Techniques in Biochemistry and Molecular Biology, vol. 15, (New York, Elsevier, 1985), pp. 9-20.
William D. Geoghegan et al., “The application of glucose oxidase-labeled antibodies for the detection of proteins on nitrocellulose”, Journal of Immunological Methods, vol. 93, (Nov. 6, 1998), pp. 231-236.
K. Kanai et al., “Variations in effectiveness of antigen retrieval pretreatments for diagnostic immunohistochemistry”, Research in Veterinary Science, vol. 64, No. 1 (Jan.-Feb. 1998), pp. 57-61.
Pádraig M. Strappe et al., “Enhancement of immunohistochemical detection of HIV-1 p24 antigen in brain by tyramida signal amplification”, Journal of Virological Methods, vol. 67 (Aug. 1997), pp. 103-112.
Emilena Toplikar et al., “Development of an Enzyme Immunoassay for the Detection of Hepatitis B Surface Antigen Employing Monoclonal Antibodies”, Journal of Clinical Laboratory Analysis, vol. 7, 1993, pp. 324-328.
Program and Abstracts, Congress of the World Veterinary Poultry Association, Jul. 19-23, Co-sponsored by the American Association of Avian Pathologists and the American Veterinary Medical Association.
North Atlantic Poultry Health and Management Conference, Mar. 20-21, 2002, Sheraton Harborside Hotel and Conference Center, Portsmouth, New Hampshire.
Proceedings of the Fifty-Second Western Poultry Disease Conference, Mar. 8-11, 2003, Sacramento, California.
Atmar, R.L., B.D. Baxter, E.A. Dominguez, L. H. Taber., “Comparison of reverse transcription-PCR with tissue culture and other rapid diagnostic assays for detection of type A influenza virus”, J Clin Microbiol. 34(10): 2604-6. 1996.
Swayne, David E., Dennis A. Senne and Charles W. Beard, “Avian Influenza”, In: A Laboratory Manual for the Identification of Avian Pathogens. Published by The American Association of Avian Pathologists, 4th ed. 1998, pp. 150-155.
Easterday, B. C., Virginia S. Hinshaw, and David A. Halvorson, “Influenza”, In: Diseases of Poultry, 10th ed, by B.W. Calnek, H.J. Bames, C. W. Beard, L.R. McDonald, and Y.M. Saif, Iowa State University Press, Ames, Iowa, pp. 583-605, 1997.
Kodihalli, S., V. Sivanandan, K.V. Nagaraja, S.M. Goyal, D.A. Halvorson, “Antigen-capture enzyme immunoassay for detection of avian influenza virus in turkeys”, Am J Vet Res. 54(9):1385-90, Sep. 1993.
Lee, M.S., P.C. Chang, J. H. Shien, M.C. Cheng, H.K. Shieh, “Identification and subtyping of avian influenza viruses by reverse transcription-PCR”, Journal of Virol Methods, 97(1-2): pp. 13-22, Sep. 2001.
Martin, S.W., Alan H. Meek, and P. Willeberg, “Veterinary Epidemiology Principles and Methods”, In Chapter 5 of Disease Causation: Table 5.3, The chi-square test (McNemar's) applied to differences between two correlated proportions. p. 127. Iowa State University Press, Ames, 1st ed. 1987.
Munich, M., L.P. Nielsen, K.J. Handberg, P.H. Jorgensen, “Detection and subtyping (H5 and H7) of avian type A influenza virus by transcription-PCR and PCR-ELISA”, Abstract, Arch Virol. 146(1):, 2001.
Pearson, J.F., and D.A. Senna, “Diagnostic procedures for avian influenza”, In Proceedings 2nd International Symposium on Avian Influenza, United States Animal Health Association, Atlanta, GA, pp. 222-227, 1986.
Pregliasco, F., C. Mensi, L. Carnorali, G. Anselimi, “Comparison of RT-PCR with other diagnostic assays for rapid detection of influenza viruses”, J Med Virol. 56(2): pp. 168.73, 1998.
Schwabe, C.W., H.P. Riemann, C.E. Franti, “Epidemilogy in Veterinary Practice”, In Chapter 5 of Mathematical Approach: Test sensitivity and specificity, pp. :74-75. Philadelphia: Lea & Febiger, 1977.
Thayer, S.G., and C.W. Beard, “Serological Procedures”, In: A Laboratory Manual for the Identification of Avian Pathogens, Published by The American Association of Avian Pathologists, 4th ed. 1998.
Waner, J.L, S.J. Todd, H. Shalaby, P. Murphy, L.V. Wall, “Comparison of Directigen FLU-A with viral isolation and direct immunofluorescence for the rapid detection and identification of influenza A virus”, J Clin Microbiol. 29(3): 479-82. 1991.
Wang, X, A.E. Castro, M.D. Castro, H. Lu, D. Weinstock, N. Soyster, W. Scheuchenzuber, M. Perdue, “Production and evaluation criteria of specific monoclonal antibodies to the hemagglutinin of the H7N2 subtype of avian influenza virus”, J. Vet Diagn Invest. 12(6): 503-9, 2000.
Webster, R.G., and C. H. Campbell, “An inhibition test for identifying the neuraminidase antigen of influenza viruses”, Avian Dis. 16: 1057-1068, 1972.
Lu, H., “A Longitudinal Study of a Novel Dot-Enzyme-Linked Immunosorbent Assay for Detection of Avian Influenza Virus”, Avian Diseases, Animal Diagnostic Laboratory, Dept. of Veterinary Science, The Penn State University, 47:362-369, 2003.
“New Lab Test for Avian Flu” ARS News Service Agricultural Research Service, USDA Sharon I Feb. 18, 2003, pp. 1-2, http:/
ps.ars.usda.gov/menu.htm?newsid=2106.
Avian Diseases “A Longitudinal Study of a Novel Dot-Enzyme-Linked Immunosorbent Assay for Detection of Avian Influenza Virus”, published by The Pennsylvania State University, Inc., Pub. Date 2003, pp. 361-369.
Avian Diseases “Investigation of H7N2 Avian Influenza Outbreaks in Two Broiler Breeder Flocks in Pennsylvania,” published by the Pennsylvania State University. Inc. Pub. 2001-2002, pp. 26-33.
Program and Abstracts, Congress of the World Veterinary Poultry Association, Jul. 19-23, Co-sponsored by the American Association of Avian Pathologists and the American Veterinary Medical Association. (2003).
McDermott Will & Emery LLP
Penn State Research Foundation
Stucker Jeffrey
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