Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical
Patent
1995-06-07
1997-03-25
Kunz, Gary L.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing compound containing saccharide radical
536 2542, C07H 100, C07H 2100
Patent
active
056143915
ABSTRACT:
A method and kit for purification of m-RNA from a cell are disclosed. Guanidine containing moieties, at high molarity, are use to quickly lyse the cell. They also act to inhibit RNase activity. Without the need for isolation of total RNA, the lysate can then be directly purified from the lysate using oligo dT (or U) by reducing the guanidine concentration via a dilution step.
REFERENCES:
K. Jakobsen et al, 18 Nuc. Acids Res. 3669 (Jun. 25, 1990).
J. Bradley et al, 6 BioTechniques 114-116 (1988).
J. Chirgwin et al, 18 Biochemistry 5294-5299 (1979).
P. Chomczynski et al, 162 Anal. Biochem. 156-159 (1987).
P. Chomcynzki et al, Cinna/Biotecx Bulletin #1 (1988).
Pharmacia LKB New Product Announcement, undated, admitted prior art "RNA Extraction Kit".
Pharmacia "RNA Extraction Kit Instruction Booklet" (1989).
Pharmacia "M-RNA Purification Kit Instruction Booklet" (1989).
H. Aviv et al, 69 P.N.A.S. USA 1408-1412 (1972).
C. Wolin et al, 16 Analects 1-4 (1988).
C. Wolin et al, 17 Analects 1, 5-7 (1989).
Thompson et al. Anal. Biochem. 181:371-378, 1989.
Pellegrino et al. Biotechniques 5(5):452-458, 1987.
Franciskovich Phillip P.
Wolin Christopher D.
Kunz Gary L.
Pharmacia P.L. Biochemicals, Inc.
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