M-RNA purification

Details M-RNA purification M-RNA purification

1994-05-02

1995-10-17

Furman, Keith C.

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

536 254, 536 2541, 435 6, 4351723, 435810, 935 4, 935 21, C12N 1510, C12Q 168, C07H 108, C07H 2102

Patent

active

054592534

ABSTRACT:
A method kit for purification of m-RNA from a cell are disclosed. Guanidine containing moieties, at high molarity, are use to quickly lyse the cell. They also act to inhibit RNase activity. Without the need for isolation of total RNA, the lysate can then be directly purified from the lysate using oligo dT (or U) by reducing the guanidine concentration via a dilution step.

REFERENCES:
Pharmacia "RNA Extraction Kit Instruction Booklet" (1989).
Pharmacia "M-RNA Purification Kit Instruction Booklet" (1989).
H. Aviv et al., 69 P.N.A.S. USA 1408-1412 (1972).
C. Wolin et al, 16 Analects 1-4 (1988).
C. Wolin et al., 17 Analects 1, 5-7 (1989).
K. Jakobsen et al., 18 Nuc. Acids Res. 3669 (Jun. 25, 1990).
J. Bradley et al., 6 BioTechniques 114-116 (1988).
J. Chirgwin et al., 18 Biochemistry 5294-5299 (1979).
P. Chomczynski et al., 162 Anal. Biochem. 156-159 (1987).
P. Chomczynski et al., Cinna/Biotecx Bulletin #1 (1988).
Pharmacia LKB New Product Announcement, admitted prior art "RNA Extraction Kit" (1989).
J. Thompson et al., Analytical Biochemistry 181:371-378 (1989).
J. Thompson et al. "A Noise-Free Molecular Hybridization Procedure for Measuring RNA in Cell Lipates" Anal. Biochem. 181:371-378 (1989).
Pellegrino, M. G. et al., "A Sensitive Solution Hybridization . . . " Biotechniques 5(5) 452-458 (1987).

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