Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving hydrolase
Patent
1979-02-02
1981-03-10
Wiseman, Thomas G.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving hydrolase
435810, 435 4, 23230B, 424 2, C12Q 134
Patent
active
042555175
ABSTRACT:
A new and improved assay for the determination of the enzyme lysozyme in biological samples which utilizes a plate assay based upon the bacterium Micrococcus lysodiekticus is described. The assay utilizes a novel composition for the assay medium which results in a reduction in assay incubation time to the point that the assay is complete after one-half hour. This reduction in time is achieved in part by the utilization of organic buffers in the assay medium and in part by the reduction of the ionic strength of the assay medium. by the use of this assay a novel technique for determining the integrity of the amniochorial membrane and maturity of fetal lungs are described.
REFERENCES:
patent: 3834991 (1974-09-01), Megraw et al.
Prasad et al., "Measurement of the Lytic Activity of Lysozymes (Muramidases)", Analytial Biochemistry, vol. 6 (1963) pp. 328-334.
Davies et al., "The Dependence of Lysozyme Activity on pH and Ionic Strength", Biophys Acta, vol. 178 (1969) pp. 294-305.
Sapse et al., "Human Tear Lysozyme", Am. J. Opthal, vol. 66 (1968) pp. 76-80.
Bonauida, et al., "Human Tear Lysozyme", Am. J. Opthal, (1968) pp. 70-76.
Ford, et al., "Identification of a Bnetericidal Factor (B-Lysin) in Amniotic Fluid at 14 and 40 Weeks Gestation", Am. J. of Obstet & Gynocol, vol. 127, pp. 788-92.
Ford, et al., "Detection of Amiotic Fluid in the Vaginal Vault by Lysozyme Concentration",copy of talk presented at the ACOG Districts VI and VII meeting on Sept. 21, 1977, San Francisco, CA.
Grossowiz, et al., "Improved Lysozyme Assay in biological Fluids",Clin. Chem. vol. 25, No. 3 (1979) pp.484-5.
Boswell K. H.
Wiseman Thomas G.
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