Multicellular living organisms and unmodified parts thereof and – Nonhuman animal – Transgenic nonhuman animal
Patent
1996-07-29
2000-09-12
Clark, Deborah J.
Multicellular living organisms and unmodified parts thereof and
Nonhuman animal
Transgenic nonhuman animal
800 7, 800 21, 119DIG1, 4241571, 424535, 424 9461, A01K 6700, A61K 39395, A61K 3520, A61K 3847
Patent
active
061180459
ABSTRACT:
The invention provides transgenic nonhuman mammals producing phosphorylated lysosomal proteins in their milk, and methods of generating the same. Phosphorylation occurs at the 6' position of a mannose side chain residue. Also provided are methods of purifying lysosomal proteins from milk, and incorporating the proteins into pharmaceutical compositions for use in enzyme replacement therapy.
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Pieper Frank R.
Reuser Arnold J. J.
Van der Ploeg Ans T.
Verbeet Martin Ph.
Academic Hospital
Clark Deborah J.
Eramus Universiteit
Pharming B.V.
The Universiteit Leiden
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