Chemistry: molecular biology and microbiology – Apparatus – Differentiated tissue perfusion or preservation apparatus
Patent
1981-12-11
1983-10-18
Lander, Ferris H.
Chemistry: molecular biology and microbiology
Apparatus
Differentiated tissue perfusion or preservation apparatus
422294, 422296, C12M 300
Patent
active
044106300
ABSTRACT:
An apparatus for blood sample treatment involves lysis, filtration and culture, the apparatus being in the form of a unitary culture chamber assembly consisting of an upper chamber for receiving a blood sample, this upper chamber receiving lysing solution squeezed from an attached bag which is subsequently detached. The upper chamber is located over and is in telescopic engagement with a lower chamber having a pointed hollow needle engageable through a rubber diaphragm in the bottom of the upper chamber when the upper chamber is pressed down. This is done after complete blood lysis, and then vacuum is applied to the lower chamber to accomplish filtration. The lower chamber is then detached and discarded. The diaphragm is sealingly covered and an attached bag of culture medium is squeezed to introduce the medium into the upper chamber from the bag. This second bag is detached, leaving the upper chamber as a complete blood culture system.
REFERENCES:
patent: 3874851 (1975-04-01), Wilkins et al.
patent: 4063460 (1977-12-01), Svensson
patent: 4119125 (1978-10-01), Elkins
patent: 4142856 (1979-03-01), Acuff
patent: 4150950 (1979-04-01), Tageguchi et al.
patent: 4185964 (1980-01-01), Lancaster
patent: 4220715 (1980-09-01), Ahnell
patent: 4252538 (1981-02-01), Barr
"Development of a Lysis-Filtration Blood Culture Technique" Journal of Clinical Microbiology, Jan. 1977, pp. 46-50.
Lander Ferris H.
The United States of America as represented by the Department of
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