Lysing reagent for leukocyte differentiation method

Details Lysing reagent for leukocyte differentiation method Lysing reagent for leukocyte differentiation method

1988-07-05

1993-01-19

Housel, James C.

Chemistry: analytical and immunological testing

Composition for standardization, calibration, simulation,...

Preparation composition

436 8, 2524081, G01N 3100

Patent

active

051806770

ABSTRACT:
A lysing reagent is employed for stromatolysing the red cells in a blood sample and differentiating the leukocytes into subpopulations. The lysing reagent contains a dodecyltrimethylammonium halide and an aromatic second cationic agent which is either a benzyltrialkylammonium halide or an alkylpyridinium halide. In some methods, the lysing reagent is mixed in the reaction chamber with a major proportion of the diluent, and the blood sample with a minor proportion of the diluent is added to the reaction chamber.

REFERENCES:
patent: 4389490 (1983-06-01), Crews et al.
patent: 4521518 (1985-06-01), Carter et al.
patent: 4656139 (1987-04-01), Matsuda et al.
"Physical and Chemical Characteristics of Armak Quaternary Ammonium Salts" Product Data Bulletin 81-6, Armak Corp., 1981.

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