Lysing reagent

Details Lysing reagent Lysing reagent

1977-02-08

1980-01-29

Marantz, Sidney

Chemistry: physical processes

Physical processes

Crystallization

252408, 424 11, G01N 3316

Patent

active

041859642

ABSTRACT:
Azide-free blood diluents and cyanide ion-free erythrocyte lysing reagents hich are particularly advantageous for use with Coulter scanning devices. The lysing reagent comprises (1) a water-soluble quaternary ammonium salt of the formula R.sub.1 N(R.sub.2).sub.3 X wherein R.sub.1 is a 12-20 C alkyl, each R.sub.2 is the same or different and is lower alkyl or lower hydroxyalkyl and X is an anion, and (2) a minor amount of a water-soluble polycarboxylic acid.

REFERENCES:
patent: 3874851 (1975-04-01), Wilkins
patent: 3874852 (1975-04-01), Hamill
patent: 3964865 (1976-06-01), Das
patent: 3977995 (1976-08-01), Louderback
patent: 4013417 (1977-03-01), Raffaele
patent: 4030888 (1977-06-01), Yamamoto
patent: 4040785 (1977-08-01), Kim
Chemical Abstracts, 70: 1593c (1969).

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