Lyophilized reagent for polymerase chain reaction

Details Lyophilized reagent for polymerase chain reaction Lyophilized reagent for polymerase chain reaction

1998-12-07

2000-11-28

Horlick, Kenneth R.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

435 911, 435 6, 435188, 536 2433, 53612313, C12P 1934, C12Q 168, C07H 2104, C12N 996

Patent

active

061534129

ABSTRACT:
The present invention provides a lyophilized reagent for PCR which is prepared by adding a stabilizing and sedimenting agent to an aqueous reaction mixture and lyophilizing thereof. The lyophilized PCR reagent of the present invention leads to a simplification of multi-step PCR manipulation, an increase of heat stability of the reaction mixture, prevention of carry-over contamination, and improved credibility of experiments. The lyophilized PCR reagent can be applied as a kit for analysis of DNA sequence or for diagnosis of diseases, which guarantee the results of high credibility in a short period of time.

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