Lyophilized reagent for polymerase chain reaction

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid

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435 912, 435188, 536 2433, 935 77, 935 78, C12P 1934, C12Q 168, C12N 996, C07H 504

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active

058612513

ABSTRACT:
The present invention provides a process for preparing a ready-to-use reagent for PCR, which comprises the steps of adding the stabilizer, glucitol or glucose, to an aqueous polymerase chain reaction(PCR) mixture containing a reaction buffer, MgCl.sub.2, dNTPs, a DNA polymerase, the water soluble dye, bromophenol blue, xylene cyanole, bromocresol red or cresol red, a primer, and optionally, ddATP, ddCTP, ddGTP, or ddTTP, and then lyophilizing thereof. The ready-to-use PCR reagent of the present invention leads to a simplification of multi-step PCR manipulation, an increase of heat stability of the reaction mixture, prevention of carry-over contamination, and improved credibility of experiments. The ready-to-use PCR reagent can be applied as a kit for analysis of DNA sequence or for diagnosis of diseases.

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