Lyophilized human hemoglobin standard for colorimetric determina

Details Lyophilized human hemoglobin standard for colorimetric determina Lyophilized human hemoglobin standard for colorimetric determina

1975-01-30

1976-06-22

Wolk, Morris O.

Chemistry: physical processes

Physical processes

Crystallization

252408, 356 39, 356 42, 356185, 424 7, G01N 2120, G01N 3316

Patent

active

039648656

ABSTRACT:
A preweighed vial of lyophilized methemoglobin (ferrihemoglobin) provides a simple, stable, reproducible standard for colorimetric determination of total hemoglobin concentration in whole blood by the cyanmethemoglobin method. Conversion of the standard and of hemoglobin in blood samples to cyanmethemoglobin is accomplished with Drabkin's solution reconstituted from a dry powder and having an added non-ionic surface active agent (Brij-35). Color intensity is measured at 540 m.mu..

REFERENCES:
harvey A. Itano et al., Medicine, 35, 131 (Feb. 1956).
Todd-Sanford Clinical Diagnosis, I. Davidson et al., eds., 14th Edition, 139, W. B. Saunders, Phila., 1969.
Gradwohl's Clinical Laboratory Methods, Sam Frankel et al., eds., vol. I, 396-406, C. V. Mosby Co., Saint Louis, 1970.

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