Lyophilized bioluminescent bacterial reagent for the detection o

Details Lyophilized bioluminescent bacterial reagent for the detection o Lyophilized bioluminescent bacterial reagent for the detection o

1996-11-22

1998-03-24

Robinson, Douglas W.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

435 6, 435 8, 4352523, 935 72, 935 73, C12N 121, C12N 1500, C12Q 166, C12Q 168

Patent

active

057311633

ABSTRACT:
A reagent, useful in the detection of environmental insults comprising bacterial cells containing a stress promoter operably linked to a lux gene complex has been prepared by lyophilizing the cells in a specified medium. The reagent may be used immediately upon rehydration where a positive test for the presence of an environmental insult is given by an increase in light production from the cells.

REFERENCES:
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Marincs et al. Immobilization of Escherichia coli expressing the lux genes of Xenorhabdus luminescens. Applied and Environmental Microbiology. 60(10):3862-3863. 1994.
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Yates, I.E. et al, "Bacterial Bioluminscence as a Bioassay for Mycotoxins", Applied & Environmental Microbiology, 44(5), 1072-1075 (1982).
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Rosson, R. A., Biluminescence for Detection of Trace Coompounds, Govt. Reports announncements & Index, Issue 09, (1990) pp. 1-7.
Park, S-F., Stewart, G.S.A.B., and Kroll, R-G, 1992, The use of Bacterial Luciferase for Monitoring the Environment Regulation of Expression of Genes Encoding Virulence Factors, in Listeria Monocytogenes J. Gen. Microbiol. 138:2619-2627.
Janda, I, and Opekarova, M. 1989, Long-term Preservation of Active Lumunous Bacteria by Lyophilization, Journal of Bioluminescence and Chemiluminescence, vol. 3:27-29.

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