Lymphatic delivery composition

Drug – bio-affecting and body treating compositions – Preparations characterized by special physical form – Particulate form

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424490, A61K 951

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active

057924759

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BRIEF SUMMARY
The present invention relates to a lymphatic delivery composition, and more particularly to a composition for delivering an active agent to the lymphatic system.
The lymphatic system has an important role in transporting body fluids and particulate materials to include proteins, fat particles, etc. Large proteins and certain cells (lymphocytes) pass from the blood plasma into the tissue fluid and it is the major function of the lymph to return these essential components to the blood circulation. The lymph also plays an important role in transporting the products of fat digestion in the gastrointestinal tract, the chylomicrons, into the blood circulation. The properties of the lymphatic system have been reviewed in detail by J. M. Yoffrey and F. C. Courtice (Lymphatics, lymph and the lymphomyeloid complex, Academic Press, London, 1970). The lymphatic system also plays and important role in the spread of tumours. Malignant cells can enter the lymphatic system and become captured by lymph nodes where secondary tumours can be produced. Eventually the whole of the lymph chain can be involved. The lymph can also be involved in the spread of tumours to other organs, for example the lungs. Consequently there is considerable need for a method of examining the lymphatic drainage and lymph nodes in the diagnosis and treatment of malignant diseases. This subject has been reviewed extensively by S. E. Strand and others (L. Bergquist et al. in Microspheres and Drug Therapy, Immunological and Medical Aspects p.263 (Edited by Davis et al.) Elsevier 1984).
It is well known that colloidal particles can have an important role in characterising the properties of the lymphatic system as well as a possible role in delivering drugs to the lymphatic system. The use of colloidal particles as radiodiagnostic agents has been reviewed by Strand et al. (Bergquist et al. Seminars in Nuclear Med. 12 (1983) 9-19). A wide range of materials has been examined to include solid particles, emulsions and vesicles (liposomes). The distribution of colloidal agents depends strongly on their particle size and colloids suggested for lymphoscintigraphy were found to have a median size of about 40-60 nm. Uptake into regional lymph nodes after, for example, subcutaneous administration is quite small and values from 1-10% are typical after 2-5 hours (Strand, S. E. CRC Crit. Rev. Drug Carrier System 6 (1989) 211-237).
A targeting system for the delivery of diagnostic and therapeutic agents to the lymphatic system should have the following characteristics: nodes.
Various attempts have been made to increase lymphatic uptake by change in particle size and particle number and particle nature and these have been reviewed by Strand (CRC Crit. Rev. Drug Carrier Systems 6 (1989) 211-237). With liposome systems the best recorded level of uptake in the lymph nodes is from 1-2% at 48 hours. This can be increased to about 5% by the attachment of antibodies. (Patel, H. M. In Liposomes as drug carriers, Ed. G. Gregoriadis, p.51 John Wiley, 1988, Kaledin, J. et al. Nat. Cancer Inst. 69 (1982) 67-71, Turner, A. et al. Biochim. Biophy. Acta 760 (1983) 119-125).
GB 2108967 describes the admixture of colloidal albumin particles with poloxamers and ethoxylated surfactant of the Cremophor series and measurements and lymphatic uptake are described. The quantities sequestered in lymph nodes are reported for times of 2 hours after administration. For the poloxamer materials only the hydrophilic materials containing 80% ethylene oxide content were examined. The uptake in the primary lymph nodes was increased with values as high as 17% reported for Poloxamer 238. For Poloxamer 188 about 13% of the colloid was captured in the primary nodes. However, only 1% was reported to be found in the secondary nodes.
High uptake into both primary and secondary lymph nodes has not been achieved and this is a big disadvantage for delivery of diagnostic and therapeutic agents to the lymphatic system.
Illum and others have described the use of surfactants and in particular the poloxamers and pol

REFERENCES:
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