Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving oxidoreductase
Patent
1986-10-27
1989-05-30
Warden, Robert J.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving oxidoreductase
435 4, 435 25, 435810, 436172, 436808, 436826, C12Q 100, C12Q 126, G01N 2176
Patent
active
048351016
ABSTRACT:
The storage stability of peroxidase-reactive luminescent cocktails which employ chemiluminescent 2,3-dihydro-1,4-phthalazinediones, oxidants reactant therewith to cause light emission in peroxidase-mediated luminescent reactions, and phenolic sensitivity enhancers is improved by maintaining the pH of the cocktail prior to use at a value in the range of about 3 to about 6 and preferably at a pH of about 5. An analyte sample may be subjected to a reaction resulting in a peroxidase-containing phase, the peroxidase content of which is related to the quantity of analyte in the sample, and the peroxidase phase may then be combined with the thus-described cocktail and an aqueous alkaline organic buffer to raise the pH of the resulting reaction mixture to a value in the range of 7-9, favoring light emission.
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Blocki Frank A.
Kao Richard
Mahoney Walter C.
Pranis Robert A.
Graeter Janelle
Haller James R.
Kallestad Diagnostics, Inc.
Warden Robert J.
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