Luminescence monitoring with modulation frequency multiplexing

Details Luminescence monitoring with modulation frequency multiplexing Luminescence monitoring with modulation frequency multiplexing

1992-01-29

1994-05-31

Sykes, Angela D.

Surgery

Truss

Pad

422 8206, 422 8207, 2504581, 2504612, A61B 500, G01N 2161

Patent

active

053159934

ABSTRACT:
A luminescence based analytical probe is excited with light varying repetitively in amplitude at multiple modulation frequencies. A composite signal incorporating components at a plurality of these modulation frequencies, produced by emissions in all of the various decaying modes is analyzed to provide information, such as the fractional luminescence contribution and/or lifetime of each individual decaying mode because the various decaying mode are segregated by analysis of the composite signal, there is no need to segregate light from the various decaying mode according to the wavelength of the light. Therefore, the probe may incorporate plural luminescent materials which have different decay modes with different luminescent decay times and sensitive to different conditions to be monitored, even if the plural luminescent materials absorb and emit light at the same wavelengths.

REFERENCES:
patent: 5000901 (1991-03-01), Iyer et al.
patent: 5102625 (1992-04-01), Hilo
patent: 5119463 (1992-06-01), Vurek et al.
patent: 5127405 (1992-07-01), Alcala et al.
"Rapid-Scanning Frequency-Domain Fluorometer With Picosecond Time Resolution," Applied Optics, vol. 26, No. 17, Sep. 1987.
"2-GHz Frequency-Domain Fluorometer," Rev. Sci. Instrum. 57(10) Oct. 1986, American Institute of Physics (1986).
"Digital Parallel Acquisition in Frequency Domain Fluorimetry", Rev. Sci. Instrum. 60(9), Sep. 1989, American Institute of Physics (1989).

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