Luminescence by reacting an acridinium ester with superoxide

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid

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C435S007100, C435S025000, C435S968000, C422S082070, C422S082080, C436S800000, C436S544000, C436S546000, C546S102000, C546S104000, C546S107000, C546S290000, C546S325000

Reissue Patent

active

RE039047

ABSTRACT:
A light emitting method of an acridinium ester, comprising reacting said acridinium ester and a superoxide anion, and a method of detecting a substance to be examined, comprising detecting a light emitted by reacting a superoxide anion with an acridinium ester used as a label am described. It is possible to carry out the reaction not under strongly alkaline conditions but around the neutral point and to generate strong luminescence which is stable over a long period of time.

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