Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving luciferase
Reexamination Certificate
2005-02-08
2005-02-08
Witz, Jean C. (Department: 1651)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving luciferase
C435S029000, C435S189000
Reexamination Certificate
active
06852499
ABSTRACT:
The present invention provides a detection method and a method of manufacturing a detection kit, both characterized by use of an organic sulfur reagent, and which are effective at low concentration of the reagent, are inexpensive, and have reduced unpleasent odor. Provided is a reagent kit for detecting aColeoptera luciferase, comprising an organic sulfur reagent having the atomic sequence of sulfur-carbon-sulfur in its chemical structure, a luciferin, adenosine triphosphate and a magnesium ion. Also provide is a method for detecting aColeoptera luciferase, comprising step 1 of mixing an aqueous solution, containing an organic sulfur reagent having the atomic sequence of sulfur-carbon-sulfur in its chemical structure, a luciferin, adenosine triphosphate and a magnesium ion, with a sample containing aColeoptera luciferase, to give a mixed solution; and step 2 of measuring the light emitted in the mixed solution.
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M. Ryufuku, et al., ITE Letters on Batteries, New Technologies & Medicine, vol. 3, No. 6, XP-009019430, pp. 713-719, “Low Amount of Diethyldithiocarbamate Enhances the Light Activity in Beetle Bioluminescence System in Vitro”, 2002.
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Ryufuku Masayuki
Suzuki Chie
Tanaka Hozumi
Hanley Susan
Oblon & Spivak, McClelland, Maier & Neustadt P.C.
Toyo B-Net Co., Ltd.
Toyo Ink Mfg. Co. Ltd.
Witz Jean C.
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