Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving luciferase
Reexamination Certificate
2008-09-16
2008-09-16
Weber, Jon P (Department: 1657)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving luciferase
C435S164000
Reexamination Certificate
active
11433791
ABSTRACT:
The invention relates to methods and kits for detecting enzyme activity using bioluminescence. In particular, it relates to a novel assay system with increased light yield for a sensitive and convenient detection of luciferase activity, such as luciferase reporter enzyme activity. Provided is a method of detecting luciferase activity in a sample, comprising incubating the sample in the presence of luciferin and ATP to allow the generation of a light signal, wherein said light signal is enhanced by performing the incubation in a reaction mixture comprising phosphate and ammonium ions, and measuring the light signal. The invention also relates to kits for use in such method.
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patent: 6503723 (2003-01-01), van Lune et al.
patent: 2004/0219622 (2004-11-01), Savage
Ito et al. Novel Bioluminscent Assay of Pyruvate Phosphate Dikianse Using Firefly Luciferase-Luciferin Reaction Ans its Application to Bioluminscent Immunoassay; Analytica Chimica Acta, vol. 421 (2000) pp. 113-120.
Ito et al., “Novel bioluminescent assay of pyruvate phosphate dikinase using firefly luciferase-luciferin reaction and its application to bioluminescent enzyme immunoassy”, Analytica Chimica Acta, 421 (2000) p. 113-120.
Bruggeman Johan Jochem
van Lune Harry
Hoffman & Baron LLP
Martin Paul C.
PerkinElmer Life and Analytical Sciences B.V.
Weber Jon P
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