Luciferase and photoprotein

Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Oxidoreductase

Reexamination Certificate

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C435S069100, C435S252300, C435S320100, C536S023200, C536S023500

Reexamination Certificate

active

07125697

ABSTRACT:
The present invention provides a polynucleotide or polynucleotides encodingOplophorusluciferase which is composed of 19 kDa and 35 kDa proteins, or the 19 kDa photoprotein, the recombinant secretionalOplophorusluciferase or the 19 kDa photoprotein encoded by the polynucleotide(s), an expression vector containing the polynucleotide(s) and a host transformed with the vector.Further, the invention provides a method for producing the recombinantOplophorusluciferase or the photoprotein.These proteins could be recombinantly produced by culturing the host cell or by in vitro translation system using the recombinant expression vector.

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