LPT2 promoter having aleurone-tissue-specific activity

Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives

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4351723, 435 691, 435 914, 800205, 536 243, 47 58, C12N 1500, C12N 504

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055257160

ABSTRACT:
The in vivo expression in the aleurone cells of a cereal of a conjugate is described. The conjugate comprises a GOI (gene of interest) and a particular Ltp (lipid transfer protein) promoter--namely the Ltp2 gene promoter. The conjugate is stably integrated within the cereal's genomic DNA.

REFERENCES:
Kalla et al. (1992) The promoter of the barley Ltp2 gene encoding an aleurone specific 7kDa lipid transfer protein contains a functional Myb binding site. EMBL/GenBank/DDBJ database.
.sup.1 McElroy et al. (1990) Isolation of an Efficient Actin Promoter for use in Rice transformation, The Plant Cell. vol. 2, pp. 163-171.
Lewin, R. (1987) When does Homology mean something else? Science vol. 237. p. 1570. and Reeck et al. (1987) "Homology" in proteins and nucleic acids: A terminology muddle and a way out. Cell vol. 50, p. 667.
Reeck et al. (1987) "homology" in proteins and nucleic acids: A terminology middle and a way out of it. Cell. vol. 50. pp. 667.
Jakobsen et al. (1989) Barley aleurone cell development: molecular coling of aleurone specific cDNA's from immature grains. Plant Molecular Biology 12, pp. 285-293.
EMBL Database Ltp2 gene sequence.
Kalla, R., et al., (1993) Characterisation of promoter elements of aleurone specific genes from barley. In Pre-Harvest Sprouting in Cereals 1992. Eds. M. K. Walker-Simmons and J. J. Ried. The American Association of Cereal Chemists, Inc., ISBN 0-913250-81-3 pp. 236-245.
Olsen, O.-A., et al. (1990) Molecular Strategies for Improving Pre-Harvest Sprouting Resistance in Cereals. In Fifth International Symposium on Pre-Harvest Sprouting in Cereals, Westview Press, pp. 92-99.

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