Chemistry: electrical and wave energy – Processes and products – Electrostatic field or electrical discharge
Patent
1991-07-17
1992-06-30
Niebling, John
Chemistry: electrical and wave energy
Processes and products
Electrostatic field or electrical discharge
204299R, G01N 2726, B01D 5702
Patent
active
051260212
ABSTRACT:
A capillary electrophoresis element is disclosed. The elements includes a capillary elctrophoresis tube containing a low-voscosity polymer solution having a selected mesh size and low-solution viscosity. The mesh size may range from 50-100 .ANG., for separating single-stranded oligonucleotides, to up to 300 .ANG. or greater, for separating relatively large duplex DNA fragments or proteins. Also disclosed is a method for formulating a low-viscosity electrophoresis separation medium having a selected mesh size.
REFERENCES:
Andreas Chrambach et al "Electrophoresis on Uncrosslinked Polyacrylamide: Molecular Sieving and its Potential Applications" Electrophoresis 7 (1986) 217-220.
S. Hjerten et al "High-Performance Electrophoresis of Acidic and Basic Low-Molecular-Weight Compounds and Proteins in the Presence of Polymers and Neutral Surfactants" Journal of Liquid Chromatography 12(13)(1989) 2471-2499.
Hans-Joachim Bode "The Use of Liquid Polyacrylamide in Electrophoresis: I. Mixed Gels Composed of Agar-Agar and Liquid Polyacrylamide" Analytical Biochemistry 83 (1977), pp. 204-210.
Hans-Joachim Bode "The Use of Liquid Polyacrylamide in Electrophoresis: II. Relationship between Gel Viscosity and Molecular Sieving" Analytical Biochemistry 83 (1977) pp. 364-371.
Toshio Takagi et al "Application of Schlieren Optics to Real-Time Monitoring of Protein Electrophoresis in Crosslinker-Free Linear Polyacrylamide Solution" Electrophoresis 12(1991) pp. 436-438.
Hans-Joachim Bode "SDS-Polyethyleneglycol Electrophoresis: A Possible Alternative to SDS-Polyacrylamide Gel Electrophoresis" FEBS Letters, vol. 65, No. 1 (May 1976) pp. 56-58.
Paul M. Horowitz et al. "Electrophoresis of Proteins and Nucleic Acids on Acrylamide-Agarose Gels Lacking Covalent Crosslinking" Analytical Biochemistry 143 (1984) pp. 333-340.
Mingde Zhu et al "Factors Affecting Free Zone Electrophoresis and Isoelectric Focusing in Capillary Electrophoresis" Journal of Chromatography 480 (1989) pp. 311-319.
Applied Biosystems Inc.
Dehlinger Peter J.
Niebling John
Smith Joseph
Starsiak Jr. John S.
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