Low-temperature inducible expression vector

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...

Reexamination Certificate

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C435S320100, C435S252300, C435S325000, C536S023100

Reexamination Certificate

active

06897042

ABSTRACT:
A vector which is characterized in containing each of the following elements:(1) a promoter which shows its action in the host to be used;(2) regulatory region for regulating the action of the promoter of (1); and(3) a region which codes for the 5′-untranslated region derived from cold-shock protein gene mRNA or a region which codes for the region where substitution, deletion, insertion or addition of at least one base is applied to the said untranslated region.

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Goldstein et al. Jan. 1990; P.N.A.S 87: 283-287.*
Journal of Bacteriology, Jun. 1992, p 3867-3873; Identification of the Protomer Region of theEschericia coliMajor Cold Shock Gene, cspA.
Molecular Microbiology (1997) 26(2), 321-335; Deletion analysis of cspA ofEschericia coli: requirement of the AT-rich UP element for cspA transcription and the downstream box in the coding region for its cold shock induction.
Sophie et al.; Analysis of theEschericia coligenome. V. DNA sequence of the reion from 76.0 to 81.5 minuete; Nucleic Acids Research, 1994, vol. 22, No. 11.
Jiang et al; The Role of the 5′-End Untranslated Region of the mRNA for CspA, the Major Cold-Shock Protein ofEscheericia coli, in Cold-Shock Adaptation; Journal of Bacteriology, Aug. 1996, vol. 178, No. 16.

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