Drug – bio-affecting and body treating compositions – Designated organic active ingredient containing – Peptide containing doai
Patent
1994-08-10
1996-10-01
Le Guyader, John L.
Drug, bio-affecting and body treating compositions
Designated organic active ingredient containing
Peptide containing doai
530362, 530363, 530418, 530419, 530470, 530829, 530830, 530364, 514 12, A61K 3838, C07K 130, C07K 134, C07K 14765
Patent
active
055611150
ABSTRACT:
Highly stable plasma-derived therapeutic albumin solutions, having a turbidity level of 5 NTU or less can be made by adding sodium caprylate to Cohn fraction II+III or IV-1 effluent at relatively low temperatures. The sodium caprylate acts as a partitioning agent to separate albumin from unwanted proteins. In preferred embodiments, the albumin source solution temperature is elevated, increased in pH and reacted for approximately six hours under conditions sufficient to disrupt the initial solution colloid, and partition albumin-containing supernatant from a colloidal disperse phase, which retains unwanted globulins and manufacturing debris. Since it tends to be a scavenger molecule, albumin is selectively stabilized by diafiltration against a buffer containing sodium caprylate, thereby assuring a high albumin monomer content and low turbidity level. The amount of sodium caprylate required for selective stabilization is determined by the amount of available binding sites on the albumin molecule.
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Bayer Corporation
Degen Nancy J.
Giblin James A.
Le Guyader John L.
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