Low pressure, shear-type cell homogenizer and method of using

Details Low pressure, shear-type cell homogenizer and method of using Low pressure, shear-type cell homogenizer and method of using

1993-10-06

1995-02-21

Eley, Timothy V.

Solid material comminution or disintegration

Processes

With cell rupturing or liberation of contained liquids

241114, 241169, 2411691, 24119912, 2412612, B09B 300, B02C 1908

Patent

active

053908597

ABSTRACT:
A method for rupturing cell walls and membranes of organic cells while leaving intra-cellular organelles substantially intact employs a culture dish for holding the cells. A pestle includes a cylindrical portion that metes with the culture dish. Extending from the cylindrical portion is an axially aligned shaft. The method includes the steps of: emplacing a layer of cells in the culture dish; placing the pestle in contact with the layer of cells; applying a pressure axially to the shaft to force the pestle into contact with the cells; and rotating the shaft and pestle a plurality of reverse-direction partial rotations to achieve a rupture of cellular membranes of cells within the culture dish. Both the base of the culture dish and the pestle are substantially planar thereby enabling uniform pressure application across the base of the culture dish.

REFERENCES:
patent: 1924 (1965-03-01), Varney
patent: 527986 (1894-10-01), Howe
patent: 2606722 (1952-08-01), Gronberg
patent: 2652202 (1953-09-01), Mallinson et al.
patent: 3556414 (1971-01-01), Eberly, Jr.
patent: 3941317 (1976-03-01), Kanor
patent: 4307846 (1981-12-01), Spelsberg
patent: 4333611 (1982-06-01), Zucker et al.
patent: 4350768 (1982-09-01), Tihon et al.
patent: 4509695 (1985-04-01), Bessman
patent: 4828395 (1989-05-01), Saito et al.
patent: 5279463 (1994-01-01), Holl

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