Low phytic acid mutants and selection thereof

Multicellular living organisms and unmodified parts thereof and – Method of introducing a polynucleotide molecule into or... – The polynucleotide alters fat – fatty oil – ester-type wax – or...

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800200, 800230, 800250, 800DIG56, 800DIG55, 800DIG57, 4351721, 47 58, 47DIG1, 47DIG9, C12N 1500, C12N 1501, A01H 100, A01H 106, A01H 500

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056890540

ABSTRACT:
Single-gene, non-lethal mutations responsible for low phytic acid-containing seeds are selectable by means of a method for assaying seeds which are otherwise phenotypically, or nearly phenotypically, normal. Maize mutants having from 20% to 95% reductions in kernel phytic acid phosphorus compared to the wild-type, without any noticeable reduction in total phosphorus, were isolated by this method. Mutants obtained in accordance with the invention are useful for developing commercial, low phytic acid seed, plant lines.

REFERENCES:
Paolo Gerbasi et al., "The HIgh Inorganic PHosphate PHenotype of Low Phytic Acid 1," abstract, 35th Annual Maize Geneticds Conference, Mar. 18-21, 1993, Pheasant Run Resort, St. Charles, IL.
Victor Raboy, "5. Biochemistry and Genetics of Phytic Acid Synthesis," Inositol Metabolism in Plants, Wiley-Liss, Inc. pub., 1990, pp. 55-76.
V. Raboy et al., "A Survey of Maize Kernel Mutants for Variation in Phytic Acid," Maydica 35: 383-390 (1990).
A. W. Jongbloed et al., "Apparent Digestible PHosphorus in the Feeding of Pigs in Relation to Availability, Requirement and Environment. 1. Digestible Phosphorus . . . " Netherlands J. Agric. Sci. 38: 567-575 (1990).
J. R. Zhou et al., "Reduction of Phytic Acid in Soybean Products Improves Zinc Bioavailability in Rats," Nutrient Requirements and Interactions, J. Nutrition 122: 2466-2473 (1992).
Victor Raboy et al., "Phytic Acid Levels in Seeds of Glycine max and and G. soja as Influenced by Phosphorus Status," Crop Science 33: 1300-1305 (1993).
Thies. 1991. Fat Sci. Technol. 93(2):49-52.
Coe et al. 1988. In Corn and Corn Improvement. Third Edition. Sprague et al., eds. Ch. 3: 81-258.
Simmonds 1979. Principles of Crop Improvement. Longman. pp. 297-303.

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