Low molecular weight extraction process

Details Low molecular weight extraction process Low molecular weight extraction process

2004-03-19

2009-06-16

Tsang, Cecilia (Department: 1654)

Chemistry: natural resins or derivatives; peptides or proteins;

Proteins, i.e., more than 100 amino acid residues

C530S399000, C530S412000, C514S002600, C514S008100, C514S012200, C534S012000, C424S085100

Reexamination Certificate

active

07547761

ABSTRACT:
A process for isolating low molecular weight (“LMW”) peptides from tissue in situ comprising the steps: a) homogenizing the tissue; b) mixing the homogenized tissue with an organic solvent to form a fully-wetted slurry; c) standing or agitating the slurry to denature the proteins in situ within the tissue; d) removing the organic solvent from the tissue; e) mixing the organic solvent-treated tissue of step d) with a sufficient volume of water or an aqueous solution to extract the peptides; f) separating a liquid extract from the tissue residue of step e) to obtain an aqueous solution containing the low molecular weight peptide fraction removed from the tissue.

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