Low-conductivity buffers for magnetic resonance measurements

Chemistry: analytical and immunological testing – Nuclear magnetic resonance – electron spin resonance or other...

Reexamination Certificate

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

C436S008000, C436S163000

Reexamination Certificate

active

06958244

ABSTRACT:
An NMR buffer is particularly useful for biomolecules that require a certain amount of salt in the buffer solution. A primary buffer component and a titrating component are selected based on low ion mobility. These selections allow for a lower conductivity buffer without reducing the salt content. As such, a higher sensitivity experiment results.

REFERENCES:
patent: 5275708 (1994-01-01), Akins, Jr. et al.
Moy et al. “High-Resolution Solution Structure of the Inhibitor-Free Catalytic Fragment of Human Fibroblast Collagenase Determined by Multidimensional NMR”, Biochemistry, 1998, v. 37, pp. 1495-1504.
Caaveiro et al. “Differential Interaction of Equinatoxin II with Model Membranes in Response to Lipid Composition ”, Biophys. J v. 80, Mar. 2001, pp. 1343-1353.
Kelly et al. “Low-Conductivity Buffers for High-Sensitivity NMR Measurements”, J. Am. Chem. Soc., 2002, v. 124, pp. 12013-12019.
“Choice of lysis buffer and additives”, http://www.embl-heidelberg.de/ExternalInfo/geerlof/draft_frames/flowchart/extraction_clarification/buffer.html, Nov. 9, 2000.
Drew et al. “Actin isoform expression, cellular heterogeneity, and contractile function in smooth muscles”, Can. J. Physiol. Pharmac., 1997, v. 75, pp. 869-877.
Ukaji et al. “Accumulation of Small Heat-Shock Protein . . . ”, Plant Physiology, Jun. 1999, v. 120, pp. 481-489.
Sudmeier, J.L. et al., “Dependence of NMR Lineshape Analysis upon Chemical Rates and Mechanisms: Implications for Enzyme Histidine Titrations”, Journal of Magnetic Resonance, vol. 40, Academic Press, Inc., 1980, pp. 377-390.
Flynn, Peter F. et al., “Optimal Use of Cryogenic Probe Technology in NMR Studies of Proteins”, American Chemical Society, vol. 122, Published on Web, 2000, pp. 4823-4824.

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Low-conductivity buffers for magnetic resonance measurements does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Low-conductivity buffers for magnetic resonance measurements, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Low-conductivity buffers for magnetic resonance measurements will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-3481701

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.