Long-chain chondroitin sugar chain and method for producing...

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical

Reexamination Certificate

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C435S170000, C435S193000, C435S252100

Reexamination Certificate

active

08067204

ABSTRACT:
A method for producing a chondroitin sugar chain comprises reacting a glucuronic acid donor, an N-acetyl galactosamine donor, a sugar receptor and a bacterial cell enzyme which synthesizes chondroitin in the presence of a surfactant. The surfactant is selected from polyoxyethylene octadecyl amine, n-decanoyl-N-methylglucamide, sodium cholate, n-octyl-β-D-thioglucopyranoside, n-nonyl-β-D-thiomaltopyranoside, sucrose monocholate, sucrose monocaprate, and sucrose monolaurate. The chondroitin sugar chain has all the following properties: a weight average molecular weight: 50,000 or more when measured by gel filtration chromatography; it is completely degraded to disaccharides with chondroitinase ABC; and when the sugar chain is decomposed with chondroitinase ABC and the decomposed products are subjected to a disaccharide analysis, substantially all of them correspond to an unsaturated disaccharide unit of chondroitin.

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Definition of surfactant from http://www.freedictionary.com/surfactatn downloaded Dec. 19, 2010.
Ninomiya, et al. “Molecular Cloning and Characterization of Chondroitin Polymerase fromEscherichia coliStrain K4,”The Journal of Biological Chemistry, vol. 277, No. 24, pp. 21567-21575, 2002.
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