Chemistry: molecular biology and microbiology – Micro-organism – per se ; compositions thereof; proces of... – Bacteria or actinomycetales; media therefor
Reexamination Certificate
2007-02-27
2007-02-27
Prouty, Rebecca E. (Department: 1652)
Chemistry: molecular biology and microbiology
Micro-organism, per se ; compositions thereof; proces of...
Bacteria or actinomycetales; media therefor
C435S120000, C435S320100, C435S252330, C435S252800, C435S254110, C536S023200
Reexamination Certificate
active
10601700
ABSTRACT:
Loline alkaloids (LA), which are 1-aminopyrrolizidines with an oxygen bridge, are produced byEpichloë(anamorph=Neotyphodium) species, endophytes of grasses. LA are insecticidal, thus helping protect host plants from insect herbivory. Suppression subtractive hybridization PCR was used to isolate transcripts up-regulated during loline alkaloid production in cultures ofNeotyphodium uncinatum. Subtracted cDNAs were cloned, and a λ-phage cDNA library from an LA-expressingN. uncinatumculture was screened with subtracted cDNA. In BLAST searches, several cDNAs identified had sequence similarities to aspartate kinases, and another with O-acetylhomoserine-(thiol)lyase. Differential expression of these two genes in LA-producing cultures ofN. uncinatumwas confirmed, and in a survey of 23 isolates from 21NeotyphodiumandEpichloëspecies these two genes strictly correlated with LA production. Two nucleic acid molecules encoding two loline alkaloid gene clusters have been identified.
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Schardl Christopher L.
Spiering Martin J.
Wilkinson Heather H.
Gebreyesus Kagnew
McDermott Will & Emery LLP
Prouty Rebecca E.
University of Kentucky Research Foundation
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