Living, fast-growing thyroid cell strain, FRTL-5

Details Living, fast-growing thyroid cell strain, FRTL-5 Living, fast-growing thyroid cell strain, FRTL-5

1983-05-31

1986-08-26

Hazel, Blondel

Chemistry: molecular biology and microbiology

Spore forming or isolating process

435948, C12N 500, C12R 191

Patent

active

046083413

ABSTRACT:
A fast growing, continuous, functional rat thyroid cell strain, FRTL-5, which maintains functional characteristics of iodide uptake and thyroglobulin synthesis over prolonged periods of culture is cloned from FRTL cells obtained from primary cultures of Fischer rat thyroid glands. The FRTL-5 cells are cultured in a medium containing approximately 5 percent calf serum supplemented with a mixture of hormones, at least one of which is thyrotropin.
The FRTL-5 cells are employed in a series of assays which measure thyroid stimulatory or inhibitory factors. The FRTL-5 system of assay specifically measures thymidine incorporation, cAMP elevation and iodide uptake and permits the evaluation of patient sera, particularly those afflicted with Graves' disease and other autoimmune thyroid diseases, thereby providing a means for determining appropriate methods of treatment.

REFERENCES:
Laccetti et al, "Ganglioside Dependent Return of TSH Receptor Function in a Rat Thyroid with a TSH Receptor Defect," Biochemical and Biophysical Research Communications 110(3) pp. 772-778, (1983).
Beguinot et al, "Structural Changes Caused by Thyrotropin in Thyroid Cells and in Liposomes Containing Reconstituted . . . " Biochemical and Biophysical Research Communications 110(1) pp. 48-54, (1983).
Avivi et al, "Adenosine 3', 5'--Monophosphate Modulated Thyrotropin Receptor Clustering and Thyrotropin Activity in Culture, " Science 214, p. 1237, (1981).
Ambesi--Impiombato, "Influence of Hormones and Serum on Growth and Differentiation of the Thyroid Cell Strain FRTL," Growth of Cells in Hormonally Defined Media, ed. Sato et al, Cold Spring Harbor Laboratory, 1982, pp. 483-492.
"Culture of Hormone--Dependent Epithelial Cells From Rat Thyroids", Proceedings of the National Academy of Science USA, 77: 3455-3459, (1980), F. S. Ambesi--Impiombato, L. A. M. Parks and H. G. Coon.

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