Living cells microencapsulated in a polymeric membrane having tw

Chemistry: molecular biology and microbiology – Carrier-bound or immobilized enzyme or microbial cell;...

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264 432, 424 931, 424408, 424459, 424460, 424462, 424491, 424497, 435177, 435178, 435182, 514963, 514970, C12N 1100, C12N 1102, C12N 1110

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056208839

ABSTRACT:
A biocompatible microcapsule containing living cells encapsulated in a membrane is disclosed. The membrane is a complex formed by the cohesion of two polymer layers. An inner layer comprises a substrate biopolymer and an outer layer comprises a synthetic polyelectrolyte having an electrolytic charge opposite that of the substrate biopolymer. Droplets of a solution of substrate biopolymer containing a suspension of living cells can be added to a solution comprising the synthetic polyelectrolyte to form the encapsulates. The membrane is formed by the cohesion of the oppositely-charge polymer layers to form a complex of substrate biopolymer and synthetic polyelectrolyte. Preferably, the inner layer contains a cationic biopolymer, such as collagen modified to have a pKI of 9, or an anionic biopolymer such as esterified or modified hyaluronic acid. Further, the membrane is permeable to materials which are able to maintain normal metabolic functions of bioactive cells which are encapsulated in the inner layer of the membrane. In addition the membrane is permeable to products released by the bioactive cells and impermeable to agents of the immune system which are responsible for immunological reactions that result in an immune rejection of bioactive cells and products thereof.

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