Living cells encapsulated in crosslinked protein

Chemistry: molecular biology and microbiology – Carrier-bound or immobilized enzyme or microbial cell;... – Enzyme or microbial cell is immobilized on or in an organic...

Patent

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

424 93, 435178, 435182, 43524022, 43524025, 4352521, C12N 1102, C12N 1110, C12N 1104, C12N 502

Patent

active

047987863

ABSTRACT:
Living cells are encapsulated by: (a) dispersing living cells in an aqueous solution of a capsule wall-forming protein; (b) forming aqueous droplets of the cell containing dispersion in an aqueous-immiscible, cell compatible continuous processing medium; and (c) crosslinking the protein with a crosslinking agent which is soluble in the continuous processing medium but substantially insoluble in the aqueous droplets. The resultant capsules containing living cells may be treated with a capsule wall degrading enzyme to enlarge pores of the capsules. The encapsulated cells may be used for administration of cells to an animal for growing the cells in the animal or for delivery to the animal of a substance produced by the cells. By the cells being encapsulated, the cells are not destroyed by the aminal immune system and immune response by the animal may be minimized.

REFERENCES:
patent: 2889252 (1959-06-01), Valentine
patent: 3137631 (1964-06-01), Soloway
patent: 3516942 (1970-06-01), Scarpelli
patent: 3642978 (1972-02-01), Ogawa
patent: 3664963 (1972-05-01), Pasin
patent: 3691090 (1972-09-01), Kitajima et al.
patent: 3714065 (1973-01-01), Kitajima et al.
patent: 3780195 (1973-12-01), Balassa
patent: 3838007 (1974-09-01), Van Velzen
patent: 3937668 (1976-02-01), Zolle
patent: 4024233 (1977-05-01), Winchell
patent: 4094965 (1978-06-01), Layne et al.
patent: 4107288 (1978-08-01), Oppenheim et al.
patent: 4147767 (1979-04-01), Yapel, Jr.
patent: 4187285 (1980-02-01), Meeks et al.
patent: 4251387 (1981-02-01), Lim et al.
patent: 4255411 (1981-03-01), Lim et al.
patent: 4257884 (1981-03-01), Lim
patent: 4324683 (1982-04-01), Lim et al.
patent: 4352883 (1982-10-01), Lim
patent: 4353888 (1982-10-01), Sefton
Chang, T. M. S., Encapsulation of Enzymes, Cell Contents, Cells, Vaccines, Antigens, Antiserum, Cofactors, Hormones and Proteins, Biomedical Applications of Immobilized Enzymes and Proteins, vol. 1, Plenum Press, 1977 (pp. 69, 83-88 & 151-153).
Chang, T. M. S., Semipermeable Microcapsules, Science, vol. 246, 1964 (pp. 524-525).
Business Week: Jan. 11, 1982, pp. 122-123.
Genetic Engineering News, "At Damon, Microencapsulated Monoclonal Antibodies", Mar./Apr. 1982 (pp. 24-25).

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Living cells encapsulated in crosslinked protein does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Living cells encapsulated in crosslinked protein, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Living cells encapsulated in crosslinked protein will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-2413388

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.