Chemistry: molecular biology and microbiology – Carrier-bound or immobilized enzyme or microbial cell;... – Enzyme or microbial cell is immobilized on or in an organic...
Patent
1982-05-06
1989-01-17
Naff, David M.
Chemistry: molecular biology and microbiology
Carrier-bound or immobilized enzyme or microbial cell;...
Enzyme or microbial cell is immobilized on or in an organic...
424 93, 435178, 435182, 43524022, 43524025, 4352521, C12N 1102, C12N 1110, C12N 1104, C12N 502
Patent
active
047987863
ABSTRACT:
Living cells are encapsulated by: (a) dispersing living cells in an aqueous solution of a capsule wall-forming protein; (b) forming aqueous droplets of the cell containing dispersion in an aqueous-immiscible, cell compatible continuous processing medium; and (c) crosslinking the protein with a crosslinking agent which is soluble in the continuous processing medium but substantially insoluble in the aqueous droplets. The resultant capsules containing living cells may be treated with a capsule wall degrading enzyme to enlarge pores of the capsules. The encapsulated cells may be used for administration of cells to an animal for growing the cells in the animal or for delivery to the animal of a substance produced by the cells. By the cells being encapsulated, the cells are not destroyed by the aminal immune system and immune response by the animal may be minimized.
REFERENCES:
patent: 2889252 (1959-06-01), Valentine
patent: 3137631 (1964-06-01), Soloway
patent: 3516942 (1970-06-01), Scarpelli
patent: 3642978 (1972-02-01), Ogawa
patent: 3664963 (1972-05-01), Pasin
patent: 3691090 (1972-09-01), Kitajima et al.
patent: 3714065 (1973-01-01), Kitajima et al.
patent: 3780195 (1973-12-01), Balassa
patent: 3838007 (1974-09-01), Van Velzen
patent: 3937668 (1976-02-01), Zolle
patent: 4024233 (1977-05-01), Winchell
patent: 4094965 (1978-06-01), Layne et al.
patent: 4107288 (1978-08-01), Oppenheim et al.
patent: 4147767 (1979-04-01), Yapel, Jr.
patent: 4187285 (1980-02-01), Meeks et al.
patent: 4251387 (1981-02-01), Lim et al.
patent: 4255411 (1981-03-01), Lim et al.
patent: 4257884 (1981-03-01), Lim
patent: 4324683 (1982-04-01), Lim et al.
patent: 4352883 (1982-10-01), Lim
patent: 4353888 (1982-10-01), Sefton
Chang, T. M. S., Encapsulation of Enzymes, Cell Contents, Cells, Vaccines, Antigens, Antiserum, Cofactors, Hormones and Proteins, Biomedical Applications of Immobilized Enzymes and Proteins, vol. 1, Plenum Press, 1977 (pp. 69, 83-88 & 151-153).
Chang, T. M. S., Semipermeable Microcapsules, Science, vol. 246, 1964 (pp. 524-525).
Business Week: Jan. 11, 1982, pp. 122-123.
Genetic Engineering News, "At Damon, Microencapsulated Monoclonal Antibodies", Mar./Apr. 1982 (pp. 24-25).
Meyers William E.
Tice Thomas R.
Naff David M.
Stolle Research and Development Corporation
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