Drug – bio-affecting and body treating compositions – Extract – body fluid – or cellular material of undetermined... – Digestive system
Patent
1992-04-09
1995-02-28
Robinson, Douglas W.
Drug, bio-affecting and body treating compositions
Extract, body fluid, or cellular material of undetermined...
Digestive system
514 2, 514 21, 530846, A61K 35407
Patent
active
053935349
ABSTRACT:
A factor has been purified from rat liver homogenate which displays preferential growth inhibitory effects on non-liver metastasizing tumor cells. The purification steps include heat treatment, ammonium sulfate precipitation, anion exchange chromatography, hydrophobic interaction chromatography, cation exchange chromatography, gel filtration chromatography, and preparative isoelectric focusing. The growth inhibitor obtained has an apparent M.sub.r of 38 kD to 40 kD and a pI of 9.1, it is not affected by reducing agents, and displays no biological TGF-b activity. This inhibitor exhibits less antiproliferative effect on liver metastasizing cells than on their non-liver metastasizing counterparts in two tumor systems: the murine RAW117 large cell lymphoma, and the human A375 melanoma.
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Cavanaugh Philip G.
Nicolson Garth L.
Board of Regents , The University of Texas System
Robinson Douglas W.
Witz Jean C.
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