Live attenuated vaccines based on cp45 HPIV-3 strain and method

Drug – bio-affecting and body treating compositions – Whole live micro-organism – cell – or virus containing – Genetically modified micro-organism – cell – or virus

Patent

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

4352351, 4353201, 435325, 435455, 514 44, A01N 6300, C12N 700, C12N 1509, C12N 500

Patent

active

061104574

ABSTRACT:
The present invention is based upon correlation of two attenuating lesions of the cp45 strain to specific genetic defects in the viral genome of cp45. Specifically, it is now understood that a significant level of attenuation of cp45 giving rise to its temperature-sensitive and cold-adapted phenotypes is directly associated with mutation of the large, or L, gene of cp45 relative to the corresponding gene in the wild-type JS strain. Moreover, it is further understood that a second attenuating lesion exits independent of the temperature-sensitive lesion, and is directly associated with mutation of the hemagglutinin-neuraminidase gene, or HN gene, of cp45 relative to the corresponding gene in the wild-type HPIV-3 (JS) strain. The correlation of these two attenuating lesions of cp45 to specific genes enables several practical applications. It is now possible to create vaccines directed at other wild-type HPIV-3 viruses and, additionally, vaccines directed at target viruses other than HPIV-3 using genetic engineering techniques. For example, the mutated L and/or HN genes of cp45 can be incorporated into the viral genome of a target virus. Alternatively, the genes of the target virus which encode its surface antigens can be incorporated into the viral genome of cp45. Moreover, it is possible to determine whether an HPIV-3 strain or a hybrid virus strain made by the methods disclosed herein is attenuated by confirming the presence or absence of mutations in its L and/or HN genes.

REFERENCES:
patent: 5869036 (1999-02-01), Belshe et al.
Strayer, D. S. The Viruses Don't Always Read the Books: Engineered Vaccines and Gene Therapy using Viral Vectors. Laboratory Investigation, vol. 71, No. 3, pp. 319-323, 1994.
Cohen, J. Bumps on the Vaccine Road. Science, vol. 265, pp. 1371-1373, Sep. 2, 1994.

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Live attenuated vaccines based on cp45 HPIV-3 strain and method does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Live attenuated vaccines based on cp45 HPIV-3 strain and method , we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Live attenuated vaccines based on cp45 HPIV-3 strain and method will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-1246661

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.