Lithium salts as red blood cell lysing and hemoglobin denaturing

Details Lithium salts as red blood cell lysing and hemoglobin denaturing Lithium salts as red blood cell lysing and hemoglobin denaturing

1992-03-30

1993-11-02

Housel, James C.

Chemistry: analytical and immunological testing

Biological cellular material tested

436 66, 436 17, 436175, 436824, 436825, 435 2, G01N 3352, G01N 3372

Patent

active

052583112

ABSTRACT:
Use of a lithium salt, particularly lithium thiocyanate, as an agent for lysing red blood cells and denaturing hemoglobin in the performance of an immunoassay to detect the relative amount of a hemoglobin derivative in a blood sample. The method is particularly useful in the determination of hemoglobin Alc. The method provides a rapid means for releasing hemoglobin from red blood cells by lysis and for exposing the epitope that is characteristic of the Alc form. Low concentrations of the lithium salt provide rapid release and denaturation of hemoglobin without the need for dilution prior to the performance of the immunoassay.

REFERENCES:
patent: 4178152 (1979-12-01), Nunogaki
patent: 4853338 (1989-08-01), Benezra et al.

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