Liquid stable triglyceride reagent system

Details Liquid stable triglyceride reagent system Liquid stable triglyceride reagent system

1985-11-14

1993-06-22

Ceperley, Mary E.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving transferase

435 19, 435 25, 435 28, 435188, 435810, 436 13, C12N 996, C12Q 144, C12Q 148

Patent

active

052216152

ABSTRACT:
A stable triglyceride assay solution for use with a chromogen system is comprised of a solution buffered with a Zwitterionic buffering agent and/or TRIS and stabilized with sortibol, gelatin, and ammonium sulfate. Solution pH is in the range of from 6.8 to 8.0. The functional enzymes are lipase, glyercol kinase, and glycerol phosphate oxidase. The preferred chromogen system is based on the inclusion of 4-aminoantipyrine for use in combination with oxidase to yield a detectable chromogen compound.

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patent: 4241178 (1980-12-01), Esders et al.
patent: 4250254 (1981-02-01), Modrovich
patent: 4368261 (1983-01-01), Klose et al.
patent: 4378429 (1983-05-01), Modrovich
patent: 4608335 (1986-08-01), Fossati
Good et al., Biochemistry, 5(2): 467-476 (1966).

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